Long non-coding RNA DSCAM-AS1 indicates a poor prognosis and modulates cell proliferation, migration and invasion in ovarian cancer via upregulating SOX4.

Abstract

OBJECTIVE:

Recent studies have revealed that long noncoding RNAs (lncRNAs) play a crucial role in tumor progression. Ovarian cancer is a common type of fatal gynecological cancer worldwide. This study aims to investigate how lncRNADSCAM-AS1 functions in the progression of ovarian cancer.

PATIENTS AND METHODS:

DSCAM-AS1 expression of both ovarian cancer cells and 56 paired of tissue samples was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Moreover, the function of DSCAM-AS1 was identified via transwell assay, wound healing assay, colony formation assay and proliferation assay in vitro. The underlying mechanism was explored through qRT-PCR and Western blot assay.

RESULTS:

DSCAM-AS1 expression was remarkably upregulated in tumor tissues compared with that in the adjacent normal tissues. Besides, ovarian cancer proliferation, migration and invasion were promoted after overexpression of DSCAM-AS1 in vitro. Moreover, after overexpression of DSCAM-AS1, SOX4 was upregulated at mRNA and protein level in vitro. Furthermore, the expression of SOX4 in tumor tissues was positively correlated with the expression of DSCAM-AS1.

CONCLUSIONS:

The above results suggested that DSCAM-AS1 can promote cell migration, invasion and proliferation in ovarian cancer by upregulating SOX4, which may offer a new therapeutic intervention for patients with ovarian cancer.

• Authors: Y Li, J Hao, Y-M Jiang, Y Liu, S-H Zhang
• Journal: European review for medical and pharmacological sciences (Eur Rev Med Pharmacol Sci) Vol. 23 Issue 10 Pg. 4143-4148 (May 2019) ISSN: 2284-0729 [Electronic] Italy
• PMID: 31173284 (Publication Type: Journal Article, Retracted Publication)

Chemical References

• RNA, Long Noncoding
• RNA, Messenger
• SOX4 protein, human
• SOXC Transcription Factors
• long noncoding RNA DSCAM-AS1, human
• Sincalide

Topics

• Cell Movement
• Cell Proliferation
• Disease Progression
• Female
• Humans
• Neoplasm Invasiveness (genetics, pathology)
• Ovarian Neoplasms (genetics, mortality)
• Prognosis
• RNA, Long Noncoding (genetics)
• RNA, Messenger (genetics)
• SOXC Transcription Factors (metabolism)
• Sincalide (metabolism)
• Up-Regulation