Oncogenic lncRNA downregulates cancer cell antigen presentation and intrinsic tumor suppression.
Abstract |
How tumor cells genetically lose antigenicity and evade immune checkpoints remains largely elusive. We report that tissue-specific expression of the human long noncoding RNA LINK-A in mouse mammary glands initiates metastatic mammary gland tumors, which phenotypically resemble human triple-negative breast cancer (TNBC). LINK-A expression facilitated crosstalk between phosphatidylinositol-(3,4,5)-trisphosphate and inhibitory G-protein-coupled receptor (GPCR) pathways, attenuating protein kinase A-mediated phosphorylation of the E3 ubiquitin ligase TRIM71. Consequently, LINK-A expression enhanced K48-polyubiquitination-mediated degradation of the antigen peptide-loading complex (PLC) and intrinsic tumor suppressors Rb and p53. Treatment with LINK-A locked nucleic acids or GPCR antagonists stabilized the PLC components, Rb and p53, and sensitized mammary gland tumors to immune checkpoint blockers. Patients with programmed ccll death protein-1(PD-1) blockade-resistant TNBC exhibited elevated LINK-A levels and downregulated PLC components. Hence we demonstrate lncRNA-dependent downregulation of antigenicity and intrinsic tumor suppression, which provides the basis for developing combinational immunotherapy treatment regimens and early TNBC prevention.
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Authors | Qingsong Hu, Youqiong Ye, Li-Chuan Chan, Yajuan Li, Ke Liang, Aifu Lin, Sergey D Egranov, Yaohua Zhang, Weiya Xia, Jing Gong, Yinghong Pan, Sujash S Chatterjee, Jun Yao, Kurt W Evans, Tina K Nguyen, Peter K Park, Jiewei Liu, Cristian Coarfa, Sri Ramya Donepudi, Vasanta Putluri, Nagireddy Putluri, Arun Sreekumar, Chandrashekar R Ambati, David H Hawke, Jeffrey R Marks, Preethi H Gunaratne, Abigail S Caudle, Aysegul A Sahin, Gabriel N Hortobagyi, Funda Meric-Bernstam, Lieping Chen, Dihua Yu, Mien-Chie Hung, Michael A Curran, Leng Han, Chunru Lin, Liuqing Yang |
Journal | Nature immunology
(Nat Immunol)
Vol. 20
Issue 7
Pg. 835-851
(07 2019)
ISSN: 1529-2916 [Electronic] United States |
PMID | 31160797
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- LINK-A long non-coding RNA, human
- RNA, Long Noncoding
- Receptors, G-Protein-Coupled
- Tumor Suppressor Protein p53
- Cyclic AMP-Dependent Protein Kinases
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Topics |
- Adenoma
(genetics, metabolism)
- Animals
- Antigen Presentation
(immunology)
- Cell Line, Tumor
- Cell Proliferation
- Cell Transformation, Neoplastic
(genetics, metabolism)
- Cyclic AMP-Dependent Protein Kinases
(metabolism)
- Disease Models, Animal
- Disease Progression
- Gene Expression Regulation, Neoplastic
- Humans
- Mice
- Neoplasms
(genetics, immunology, metabolism, pathology)
- Oncogenes
- Phosphorylation
- RNA, Long Noncoding
(genetics)
- Receptors, G-Protein-Coupled
(antagonists & inhibitors)
- Tumor Escape
(genetics, immunology)
- Tumor Microenvironment
(genetics, immunology)
- Tumor Suppressor Protein p53
(metabolism)
- Ubiquitination
- Xenograft Model Antitumor Assays
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