Abstract | BACKGROUND: MATERIALS AND METHODS: RESULTS: The urinary microalbumin/ creatinine ratio and serum creatinine level were higher in diabetic nephropathy patients, and the estimated glomerular filtration rate (eGFR) was significantly decreased compared to that in diabetic patients and healthy controls (< 0.05). Compared with healthy controls, 245 upregulated and 680 downregulated lncRNAs were identified in the serum of diabetic patients, and 45 and 813 lncRNAs were up- and downregulated in the serum of diabetic nephropathy patients compared with diabetic patients. Levels of lncRNA-ARAP1-AS2 gradually increased during the progression of diabetes and diabetic nephropathy (2.82 times in DM/N and 2.47 times in DN/DM), whereas those of lncRNA-ARAP1-AS1 gradually decreased (2.24 times in DM/N, 4.79 times in DN/DM). Additionally, mRNA levels of their target gene ARAP1 (ArfGAP with RhoGAP domain, ankyrin repeat, and PH domain 1) gradually increased (2.25 times in DM/N and 2.45 times in DN/DM). CONCLUSION: lncRNA-ARAP1-AS1 and ARAP1-AS2 enhanced ARAP1 mRNA expression and may be involved in the pathogenesis of diabetes and DN. Circulating lncRNA-ARAP1-AS1, ARAP1-AS2, and ARAP1 may serve as new biomarkers for diabetes and diabetic nephropathy.
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Authors | Ying Yang, Xiaomeng Lv, Qiuling Fan, Xu Wang, Li Xu, Xinxing Lu, Tong Chen |
Journal | Clinical nephrology
(Clin Nephrol)
Vol. 92
Issue 1
Pg. 25-35
(Jul 2019)
ISSN: 0301-0430 [Print] Germany |
PMID | 31079598
(Publication Type: Journal Article)
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Chemical References |
- ARAP1 protein, human
- Biomarkers
- Carrier Proteins
- GTPase-Activating Proteins
- RNA, Long Noncoding
- RNA, Messenger
- Creatinine
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Topics |
- Adult
- Albuminuria
(urine)
- Biomarkers
(blood)
- Carrier Proteins
(genetics)
- Case-Control Studies
- Creatinine
(blood, urine)
- Diabetes Mellitus
(blood, genetics)
- Diabetic Nephropathies
(blood, genetics)
- Disease Progression
- Down-Regulation
- Female
- GTPase-Activating Proteins
(genetics)
- Gene Expression
- Glomerular Filtration Rate
- Humans
- Male
- Middle Aged
- RNA, Long Noncoding
(blood, genetics)
- RNA, Messenger
(blood)
- Up-Regulation
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