Staphylococcus saprophyticus is a gram-positive
coagulase negative bacteria which shows clinical importance due to its capability of causing
urinary tract infections (UTI), as well as its ability to persist in this environment. Little is known about how S. saprophyticus adapts to the pH shift that occurs during
infection. Thus, in this study we aim to use a proteomic approach to analyze the metabolic adaptations which occur as a response by S. saprophyticus when exposed to
acid (5.5) and alkaline (9.0) pH environments.
Proteins related to
iron storage are overexpressed in
acid pH, whilst
iron acquisition
proteins are overexpressed in alkaline pH. It likely occurs because
iron is soluble at
acid pH and insoluble at alkaline pH. To evaluate if S. saprophyticus synthesizes
siderophores, CAS assays were performed, and the results confirmed their production. The chemical characterization of
siderophores demonstrates that S. saprophyticus produces carboxylates derived from
citrate. Of special note is the fact that
citrate synthase (CS) is down-regulated during incubation at
acid pH, corroborating this result. This data was also confirmed by enzymatic assay. Our results demonstrate that
iron metabolism regulation is influenced by different pH levels, and show, for the first time, the production of
siderophores by S. saprophyticus. Enzymatic assays suggest that
citrate from the tricarboxylic acid cycle (TCA) is used as substrate for
siderophore production.