Simultaneous quantitative analysis of phosphocreatine, creatine and creatinine in plasma of children by HPLC-MS/MS method: Application to a pharmacokinetic study in children with viral myocarditis.

Abstract

A simple and rapid HPLC-MS/MS method was developed and validated for simultaneous measurement of phosphocreatine and its metabolites creatine and creatinine in children's plasma. A 50 μL aliquot of plasma was prepared by protein precipitation with acetonitrile-water (1000 μL, 1:1, v/v) followed by separation on a Hypersil Gold C18 column (35°C) with gradient mobile phase consisting of 2 mm ammonium acetate aqueous solution (pH 10) and methanol at a flow rate of 0.3 mL/min and analyzed by mass spectrometry in both positive (phosphocreatine) and negative (creatine and creatinine) ion multiple reaction monitoring mode. Good linearity (r > 0.99) was obtained for the three analytes. The intra-day and inter-day values of CV were <5.46% (-13.09% ≤ RE ≤ 2.57%). The average recoveries of the three analytes were 70.9-97.5%. No obvious impact was found for the quantitation of three analytes in normal, hemolyzed and hyperlipemic plasma. In the end, this method was successfully applied to a pharmacokinetic study of phosphocreatine in children (six cases) with viral myocarditis of children after intravenous infusion of 2 g of the test drug. The pharmacokinetc parameters of phosphocreatine/creatine were as follows: t1/2 0.24/0.83 h, Tmax 0.49/0.55 h, Cmax 47.34/59.29 μg/mL, AUClast 17.07/59.63 h μg/mL, AUCinf 17.16/79.01 h μg/mL and MRT 0.29/0.67 h.

• Authors: Ning Sun, Qirui Li, Libo Zhao, Huan He, Meng Zhang, Xiaoling Wang
• Journal: Biomedical chromatography : BMC (Biomed Chromatogr) Vol. 33 Issue 8 Pg. e4558 (Aug 2019) ISSN: 1099-0801 [Electronic] England
• PMID: 31013362 (Publication Type: Journal Article)
• Copyright: © 2019 John Wiley & Sons, Ltd.

Chemical References

• Phosphocreatine
• Creatinine
• Creatine

Topics

• Adolescent
• Child
• Chromatography, High Pressure Liquid (methods)
• Creatine (blood, chemistry, pharmacokinetics)
• Creatinine (blood, chemistry, pharmacokinetics)
• Drug Stability
• Female
• Humans
• Limit of Detection
• Linear Models
• Male
• Myocarditis (blood)
• Phosphocreatine (blood, chemistry, pharmacokinetics)
• Reproducibility of Results
• Tandem Mass Spectrometry (methods)
• Virus Diseases (blood)