MicroRNA-182-5p Modulates Oral Squamous Cell Carcinoma Migration and Invasion Via Targeting MTSS1 Gene.

Abstract

Oral squamous cell carcinoma (OSCC) characterized with invasive growth, local metastasis and later stage diagnosis was a common malignancy in head and neck region. The aim of this study was to explore the relationship between miR-182-5p and OSCC, which will contribute to find potential biomarker for OSCC metastasis. MiR-182-5p expression level was detected by the quantitative real-time PCR (qRT-PCR). Cell migration and invasion ability were examined by scratch and transwell assay. Loss-of function together with luciferase reporter assay were used to verify the miR-182-5p modulated OSCC cells migration and metastasis was mediated by MTSS1. The expression of MTSS1 protein was examined by western blotting. MiR-182-5p up-regulated in OSCC, was involved in the migration and invasion of OSCC and the increased miR-182-5p expression was correlated with lower OSCC differentiation grade, higher T and N stage. Bioinformatics analysis predicted MTSS1 gene was a potential target of miR-182-5p. Following co-transfection, qRT-PCR, luciferase activities assay and western blotting confirmed that MTSS1 gene was a direct target of miR-182-5p and silence of MTSS1 could reverse the effects of miR-182-5p on OSCC migration and invasion. MiR-182-5p was up-regulated in OSCC and the ability of miR-182-5p to promote MTSS1 repression may precipitate in the OSCC through bypassing cell migration and invasion control.

• Authors: Yan Guo, Xue Qiao, Li Zhu, Rongbo Song
• Journal: Pathology oncology research : POR (Pathol Oncol Res) Vol. 26 Issue 2 Pg. 1007-1013 (Apr 2020) ISSN: 1532-2807 [Electronic] Switzerland
• PMID: 30949866 (Publication Type: Journal Article)

Chemical References

• MTSS1 protein, human
• MicroRNAs
• Microfilament Proteins
• Mirn182 microRNA, human
• Neoplasm Proteins

Topics

• Adult
• Cell Movement (genetics)
• Female
• Gene Expression Regulation, Neoplastic (genetics)
• Head and Neck Neoplasms (genetics, pathology)
• Humans
• Male
• MicroRNAs (genetics)
• Microfilament Proteins (genetics)
• Middle Aged
• Neoplasm Invasiveness (genetics)
• Neoplasm Proteins (genetics)
• Squamous Cell Carcinoma of Head and Neck (genetics, pathology)