Abstract |
A new simple and robust HPLC-DAD method was developed for the concurrent analysis of hydroquinone (HQ), hydrocortisone acetate (HCA) and tretinoin (TRN) triple combination for the first time using an Inertsil ODS 3-C18 column (150 mm × 4.6 mm, 5 μm particle size) column with 0.05 M phosphate buffer (pH 5.0) and acetonitrile at a ratio of (10:90, v/v) as a mobile phase, eluted by an isocratic elution mode at a flow rate of 1.0 mL/min and detected at 265 nm. Mefenamic acid was used as an internal standard (I.S.). The method produced linear responses in the concentration range of 10-200, 5-100 and 1-40 μg/mL, with detection limits of 2.01, 1.13 and 0.28 × 10-3 and quantitation limits of 6.11, 3.41 and 0.87 × 10-3 μg/mL for HQ, HCA and TRN, respectively, and a correlation coefficient higher than 0.9998. All validation requirements were satisfied by proving its linearity, precision, accuracy, robustness and specificity. The method was extended for application in triple combination cream, HQ/TRN co-formulated cream and HQ and TRN single ingredient cream with a recovery >97%.
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Authors | Fawzia Ibrahim, Mohie K Sharaf El-Din, Asmaa Kamal El-Deen, Kuniyoshi Shimizu |
Journal | Journal of chromatographic science
(J Chromatogr Sci)
Vol. 57
Issue 6
Pg. 495-501
(Jul 01 2019)
ISSN: 1945-239X [Electronic] United States |
PMID | 30941396
(Publication Type: Journal Article)
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Copyright | © The Author(s) 2019. Published by Oxford University Press. All rights reserved. For Permissions, please email: [email protected]. |
Chemical References |
- Dermatologic Agents
- Drug Combinations
- Hydroquinones
- Mefenamic Acid
- hydrocortisone acetate
- Tretinoin
- Hydrocortisone
- hydroquinone
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Topics |
- Chromatography, High Pressure Liquid
(methods)
- Dermatologic Agents
(analysis)
- Drug Combinations
- Humans
- Hydrocortisone
(analogs & derivatives, analysis)
- Hydroquinones
(analysis)
- Limit of Detection
- Linear Models
- Mefenamic Acid
- Melanosis
- Reproducibility of Results
- Tretinoin
(analysis)
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