Damage on plants activates Ca2+-dependent metacaspases for release of immunomodulatory peptides.
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| Abstract |
Physical damage to cells leads to the release of immunomodulatory peptides to elicit a wound defense response in the surrounding tissue. In Arabidopsis thaliana, the plant elicitor peptide 1 (Pep1) is processed from its protein precursor, PRECURSOR OF PEP1 (PROPEP1). We demonstrate that upon damage, both at the tissue and single-cell levels, the cysteine protease METACASPASE4 (MC4) is instantly and spatiotemporally activated by binding high levels of Ca2+ and is necessary and sufficient for Pep1 maturation. Cytosol-localized PROPEP1 and MC4 react only after loss of plasma membrane integrity and prolonged extracellular Ca2+ entry. Our results reveal that a robust mechanism consisting of conserved molecular components links the intracellular and Ca2+-dependent activation of a specific cysteine protease with the maturation of damage-induced wound defense signals.
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| Authors | Tim Hander, Álvaro D Fernández-Fernández, Robert P Kumpf, Patrick Willems, Hendrik Schatowitz, Debbie Rombaut, An Staes, Jonah Nolf, Robin Pottie, Panfeng Yao, Amanda Gonçalves, Benjamin Pavie, Thomas Boller, Kris Gevaert, Frank Van Breusegem, Sebastian Bartels, Simon Stael |
| Journal | Science (New York, N.Y.) (Science) Vol. 363 Issue 6433 (Mar 22 2019) ISSN: 1095-9203 [Electronic] United States |
| PMID | 30898901 (Publication Type: Journal Article) |
| Copyright | Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. |
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