Ectopic expression of human airway trypsin-like protease 4 in acute myeloid leukemia promotes cancer cell invasion and tumor growth.

Abstract

Transmembrane serine proteases have been implicated in the development and progression of solid and hematological cancers. Human airway trypsin-like protease 4 (HAT-L4) is a transmembrane serine protease expressed in epithelial cells and exocrine glands. In the skin, HAT-L4 is important for normal epidermal barrier function. Here, we report an unexpected finding of ectopic HAT-L4 expression in neutrophils and monocytes from acute myeloid leukemia (AML) patients. Such expression was not detected in bone marrow cells from normal individuals or patients with chronic myeloid leukemia, acute lymphocytic leukemia and chronic lymphocytic leukemia. In AML patients who underwent chemotherapy, persistent HAT-L4 expression in bone marrow cells was associated with minimal residual disease and poor prognostic outcomes. In culture, silencing HAT-L4 expression in AML-derived THP-1 cells by short hairpin RNAs inhibited matrix metalloproteinase-2 activation and Matrigel invasion. In mouse xenograft models, inhibition of HAT-L4 expression reduced the proliferation and growth of THP-1 cell-derived tumors. Our results indicate that ectopic HAT-L4 expression is a pathological mechanism in AML and that HAT-L4 may be used as a cell surface marker for AML blast detection and targeting.

• Authors: Ruhong Yan, Meng Liu, Yae Hu, Lina Wang, Can Wang, Yizhi Jiang, Quansheng Zhou, Xiaofei Qi, Ningzheng Dong, Qingyu Wu
• Journal: Cancer medicine (Cancer Med) Vol. 8 Issue 5 Pg. 2348-2359 (05 2019) ISSN: 2045-7634 [Electronic] United States
• PMID: 30843660 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2019 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.

Chemical References

• Membrane Proteins
• Serine Proteases
• TMPRSS11F protein, human
• MMP2 protein, human
• Matrix Metalloproteinase 2

Topics

• Animals
• Cell Line, Tumor
• HL-60 Cells
• HeLa Cells
• Humans
• Jurkat Cells
• K562 Cells
• Leukemia, Myeloid, Acute (genetics, metabolism, pathology)
• Male
• Matrix Metalloproteinase 2 (metabolism)
• Membrane Proteins (genetics, metabolism)
• Mice
• Monocytes (metabolism)
• Neoplasm Transplantation
• Neutrophils (metabolism)
• Serine Proteases (genetics, metabolism)
• THP-1 Cells