Expression of Snail and E-cadherin in Drug-resistant MCF-7/ADM Breast Cancer Cell Strains.

Abstract

OBJECTIVE:

To investigate the expression of zinc finger transcription factors-Snail and E-cadherin in adriamycin-resistant human breast cancer MCF-7/ADM cells and non-resistant MCF-7 cells.

STUDY DESIGN:

An experimental study.

PLACE AND DURATION OF STUDY:

The Affiliated Cancer Hospital of Nanjing Medical University and Jiangsu Cancer Hospital, and Jiangsu Institute of Cancer Research, China, from April 2017 to March 2018.

METHODOLOGY:

Real-time quantitative PCR technology was used to detect the expression levels of Snail mRNA and E-cadherin mRNA in normal breast cells, adriamycin-resistant human breast cancer MCF-7/ADM cells and non-resistant MCF-7 cells. Western blot was used to detect the expression levels of proteins of Snail and E-cadherin in normal breast cells, adriamycin-resistant human breast cancer MCF-7/ADM cells and non-resistant MCF-7 cells.

RESULTS:

The expression of Snail mRNA and protein in adriamycin-resistant human breast cancer MCF-7/ADM cells was significantly higher than that in normal breast cells (p<0.001) and non-resistant MCF-7 cells (p<0.001). The expression of E-cadherin mRNA and protein in adriamycin-resistant human breast cancer MCF-7/ADM cells was significantly lower than that in normal breast cells (p<0.001) and non-resistant MCF-7 cells (p<0.001).

CONCLUSION:

Adriamycin-resistant human breast cancer MCF-7/ADM cell strains had high expression of Snail and low expression of E-cadherin. This points out to a new research direction for the targeted therapy of drug-resistant breast cancer cells, and provides clinical guidance for breast cancer therapy and prognosis evaluation.

• Authors: Fenglin Cai, Hualin Xiao, Yuting Sun, Dongfeng Wang, Jinhai Tang
• Journal: Journal of the College of Physicians and Surgeons--Pakistan : JCPSP (J Coll Physicians Surg Pak) Vol. 29 Issue 3 Pg. 240-244 (Mar 2019) ISSN: 1681-7168 [Electronic] Pakistan
• PMID: 30823950 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Cadherins
• RNA, Messenger
• SNAI1 protein, human
• Snail Family Transcription Factors
• Doxorubicin

Topics

• Blotting, Western
• Breast Neoplasms (genetics, pathology)
• Cadherins (genetics)
• China
• Doxorubicin (pharmacology)
• Drug Resistance, Neoplasm
• Female
• Gene Expression Regulation, Neoplastic
• Hospitals, University
• Humans
• MCF-7 Cells (drug effects, metabolism)
• Molecular Targeted Therapy
• RNA, Messenger (genetics)
• Real-Time Polymerase Chain Reaction (methods)
• Reference Values
• Snail Family Transcription Factors (genetics)
• Tumor Cells, Cultured (drug effects)