Apoptosis Induced by Ultraviolet A Exposure in the Presence of Enoxacin in HL-60 Cells.

Abstract

BACKGROUND:

The ultraviolet A (UVA) spectrum mainly includes the region associated with the phototoxicity of fluoroquinolone antimicrobial agents. This study investigated apoptosis induced with UVA light and enoxacin in HL-60 cells.

MATERIALS AND METHODS:

HL-60 cells were irradiated by UVA (1.1 mW/cm2) for 20 min in the presence or absence of enoxacin. The induction of apoptosis was investigated by analysing cell morphology, flow cytometry of annexin V-positive cells, DNA ladder formation, and caspase-3 activation.

RESULTS:

Significant induction of apoptosis, DNA fragmentation, and caspase-3 activation were observed in cells treated with both UVA and enoxacin. UVA-induced apoptosis was significantly suppressed when NaN3, a singlet oxygen scavenger, was present.

CONCLUSION:

Apoptosis was induced by the combination of UVA and enoxacin in HL-60 cells, and singlet oxygen appears to play an important role in photodynamically-induced apoptosis.

• Authors: Haruka Shinada, Takahiro Watanabe, Kazuho Okudaira, Yumiko Iwase, Koji Nishi, Nagahiko Yumita
• Journal: Anticancer research (Anticancer Res) Vol. 39 Issue 2 Pg. 687-693 (Feb 2019) ISSN: 1791-7530 [Electronic] Greece
• PMID: 30711946 (Publication Type: Journal Article)
• Copyright: Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Chemical References

• Fluoroquinolones
• Free Radical Scavengers
• Photosensitizing Agents
• Reactive Oxygen Species
• Enoxacin
• DNA
• Sodium Azide
• CASP3 protein, human
• Caspase 3

Topics

• Apoptosis
• Caspase 3 (metabolism)
• DNA (drug effects, radiation effects)
• DNA Fragmentation (drug effects)
• Enoxacin (pharmacology)
• Flow Cytometry
• Fluoroquinolones (pharmacology)
• Free Radical Scavengers
• HL-60 Cells
• Humans
• Photosensitizing Agents (chemistry)
• Reactive Oxygen Species
• Sodium Azide (chemistry)
• Ultraviolet Rays