Biofabrication of nano copper oxide and its aptamer bioconjugate for delivery of mRNA 29b to lung cancer cells.

Abstract

Copper oxide nanoparticles (CuO NPs) are fabricated using Coleus aromaticus leaf extract with an environmental friendly method and studied using various microscopic and spectroscopic techniques. Also, a new aptamer-conjugated hybrid delivery system using green synthesized CuO NPs is developed to deliver miRNA-29b to A549 cells. This delivery system can effectively deliver miRNAs to cancer cells, with superior performance compared to traditionally available transfection agents, thus acting as an efficient platform for intracellular miRNA delivery and improving therapeutic outcomes for lung cancer.

• Authors: Duoguang Wu, Wenjian Wang, Xiaotian He, Ming Jiang, Cuiping Lai, Xueting Hu, Jingle Xi, Minghui Wang
• Journal: Materials science & engineering. C, Materials for biological applications (Mater Sci Eng C Mater Biol Appl) Vol. 97 Pg. 827-832 (Apr 2019) ISSN: 1873-0191 [Electronic] Netherlands
• PMID: 30678973 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier B.V. All rights reserved.

Chemical References

• Aptamers, Nucleotide
• MIRN29a microRNA, human
• MicroRNAs
• Plant Extracts
• Copper
• cuprous oxide

Topics

• A549 Cells
• Aptamers, Nucleotide (chemistry)
• Cell Survival (drug effects)
• Coleus (chemistry, metabolism)
• Copper (chemistry)
• Dynamic Light Scattering
• Green Chemistry Technology
• Humans
• Lung Neoplasms (metabolism, pathology)
• Metal Nanoparticles (chemistry, toxicity)
• MicroRNAs (chemistry, metabolism)
• Microscopy, Fluorescence
• Plant Extracts (chemistry)
• Transfection (methods)