Abstract | BACKGROUND: METHODS: We compared phosphoprotein profiles of HCAECs stimulated by the serum of KD patients and normal children using iTRAQ technology, TiO2 enrichment phosphorylated peptide and MS analysis. Then we conducted the functional analysis by ClueGO and the biological interaction networking analysis by ReactomeFIViz. Western blotting was performed to identify the hub proteins. RESULTS: Our results revealed that phosphorylation of 148 proteins showed different intensities between the two HCAECs groups, which are enriched in MAPK, VEGFR, EGFR, Angiopoietin receptor, mTOR, FAK signaling pathway and so on. Through the Network Analyzer analysis, the hub proteins are CDKN1A, MAPK1 and POLR2A, which were experimentally validated. CONCLUSION: In summary, we provided evidence addressing the valuable phosphorylation signaling that could be useful resource to understand the molecular mechanism and the potential targets for novel therapy of KD.
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Authors | Shui-Ming Li, Wan-Ting Liu, Fang Yang, Qi-Jian Yi, Shuai Zhang, Hong-Ling Jia |
Journal | BMC cardiovascular disorders
(BMC Cardiovasc Disord)
Vol. 19
Issue 1
Pg. 21
(01 17 2019)
ISSN: 1471-2261 [Electronic] England |
PMID | 30654760
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Case-Control Studies
- Cells, Cultured
- Child, Preschool
- Chromatography, High Pressure Liquid
- Chromatography, Reverse-Phase
- Coronary Vessels
(metabolism)
- Endothelial Cells
(metabolism)
- Female
- Humans
- Infant
- Male
- Mucocutaneous Lymph Node Syndrome
(blood, diagnosis)
- Phosphorylation
- Protein Interaction Maps
- Proteins
(metabolism)
- Proteomics
(methods)
- Signal Transduction
- Spectrometry, Mass, Electrospray Ionization
- Tandem Mass Spectrometry
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