Counteraction of perforated cecum lesions in rats: Effects of pentadecapeptide BPC 157, L-NAME and L-arginine.

Abstract

AIM:

To study the counteraction of perforated cecum lesion using BPC 157 and nitric oxide (NO) system agents.

METHODS:

Alongside with the agents' application (after 1 min, medication (/kg, 10 mL/2 min bath/rat) includes: BPC 157 (10 μg), L-NAME (5 mg), L-arginine (100mg) alone or combined, and saline baths (controls)) on the rat perforate cecum injury, we continuously assessed the gross reappearance of the vessels (USB microcamera) quickly propagating toward the defect at the cecum surface, defect contraction, bleeding attenuation, MDA- and NO-levels in cecum tissue at 15 min, and severity of cecum lesions and adhesions at 1 and 7 d.

RESULTS:

Post-injury, during/after a saline bath, the number of vessels was significantly reduced, the defect was slightly narrowed, bleeding was significant and MDA-levels increased and NO-levels decreased. BPC 157 bath: the vessel presentation was markedly increased, the defect was noticeably narrowed, the bleeding time was shortened and MDA- and NO-levels remained normal. L-NAME: reduced vessel presentation but not more than the control, did not change defect and shortened bleeding. L-arginine: exhibited less vessel reduction, did not change the defect and prolonged bleeding. In combination, mutual counteraction occurred (L-NAME + L-arginine) or the presentation was similar to that of BPC 157 rats (BPC 157 + L-NAME; BPC 157 + L-arginine; BPC 157 + L-NAME + L-arginine), except the defect did not change. Thereby at day 1 and 7, saline, L-NAME, L-arginine and L-NAME + L-arginine failed (defect was still open and large adhesions present).

CONCLUSION:

The therapeutic effect was achieved with BPC 157 alone or in combination with L-NAME and L-arginine as it was able to consolidate the stimulating and inhibiting effects of the NO-system towards more effective healing recruiting vessels.

• Authors: Domagoj Drmic, Mariam Samara, Tinka Vidovic, Dominik Malekinusic, Marko Antunovic, Borna Vrdoljak, Jelena Ruzman, Marija Milkovic Perisa, Katarina Horvat Pavlov, Jerusha Jeyakumar, Sven Seiwerth, Predrag Sikiric
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 24 Issue 48 Pg. 5462-5476 (Dec 28 2018) ISSN: 2219-2840 [Electronic] United States
• PMID: 30622376 (Publication Type: Journal Article)

Chemical References

• Anti-Ulcer Agents
• Peptide Fragments
• Proteins
• Nitric Oxide
• BPC 157
• Arginine
• NG-Nitroarginine Methyl Ester

Topics

• Animals
• Anti-Ulcer Agents (pharmacology, therapeutic use)
• Arginine (pharmacology, therapeutic use)
• Cecum (blood supply, drug effects, injuries)
• Disease Models, Animal
• Drug Therapy, Combination (methods)
• Humans
• Intestinal Perforation (drug therapy)
• Male
• NG-Nitroarginine Methyl Ester (pharmacology, therapeutic use)
• Nitric Oxide (metabolism)
• Peptide Fragments (pharmacology, therapeutic use)
• Proteins (pharmacology, therapeutic use)
• Rats
• Rats, Wistar
• Treatment Outcome
• Wound Healing (drug effects)