Abstract | OBJECTIVE: To investigate paraffin section thickness in immunohistochemical detection of p16 expression in cervical tissue samples. METHODS: RESULTS: With the increase of slice thickness, the staining of p16 protein in nucleus gradually increased. The thickness of cervical slices in chronic cervicitis and cervical cancer samples had no significant effect on the positive rate of p16 protein(χ2=7.817 and 1.332, both P>0.05), while the thickness of slices in LSIL and HSIL samples had significant effect on the positive rate of p16 protein (χ2=17.688 and 10.182, P<0.05 or P<0.01). The stable and reliable results were obtained when the slices were between 3 and 5 μm thick. CONCLUSIONS:
Paraffin sections with thickness of 3.0-5.0 μm are recommended for immnohistochemical staining of p16 protein in cervical tissue samples.
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Authors | Zhiqiang Chen, Xianjun Mi, Ang Chen, Lifeng Duan, Xinzhen Dai, Wentong Deng |
Journal | Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences
(Zhejiang Da Xue Xue Bao Yi Xue Ban)
Vol. 47
Issue 4
Pg. 362-366
(02 25 2018)
ISSN: 1008-9292 [Print] China |
PMID | 30511522
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- Cyclin-Dependent Kinase Inhibitor p16
- Paraffin
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Topics |
- Biomarkers, Tumor
(genetics, metabolism)
- Cyclin-Dependent Kinase Inhibitor p16
(genetics, metabolism)
- Female
- Histocytological Preparation Techniques
(standards)
- Humans
- Immunohistochemistry
- Paraffin
- Squamous Intraepithelial Lesions of the Cervix
- Uterine Cervical Neoplasms
(physiopathology)
- Uterine Cervical Dysplasia
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