Abstract | BACKGROUND/OBJECTIVES: METHODS: From a human pancreatic cancer cell line, MIA PaCa-2, MIA PaCa-2-A cells with an epithelial morphology and MIA PaCa-2-R cells with a non-epithelial morphology were clonogenically isolated by the limiting dilution method. Gene expression of these subpopulations was analyzed by DNA microarray. Gene knockdown was performed using siRNA. RESULTS: Although the MIA PaCa-2-A and MIA PaCa-2-R cells displayed the same DNA short tandem repeat (STR) pattern identical to that of the parental MIA PaCa-2 cells, the MIA PaCa-2-A cells were more proliferative than the MIA PaCa-2-R cells both in culture and in tumor xenografts generated in immunodeficient mice. Furthermore, the MIA PaCa-2-A cells were more resistant to gemcitabine than the MIA PaCa-2-R cells. DNA microarray analysis revealed a high expression of claudin (CLDN) 7 in the MIA PaCa-2-A cells, as opposed to a low expression in the MIA PaCa-2-R cells. The knockdown of CLDN7 in the MIA PaCa-2-A cells induced a marked inhibition of proliferation. The MIA PaCa-2-A cells in which CLDN7 was knocked down exhibited a decreased expression of phosphorylated extracellular signal-regulated kinase (p-Erk)1/2 and G1 cell cycle arrest. CONCLUSIONS: CLDN7 may be expressed in the rapidly proliferating and dominant cell population in human pancreatic cancer tissues and may be a novel molecular target for the treatment of pancreatic cancer.
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Authors | Norimitsu Okui, Yuko Kamata, Yukiko Sagawa, Akiko Kuhara, Kazumi Hayashi, Tadashi Uwagawa, Sadamu Homma, Katsuhiko Yanaga |
Journal | Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]
(Pancreatology)
Vol. 19
Issue 1
Pg. 88-96
(Jan 2019)
ISSN: 1424-3911 [Electronic] Switzerland |
PMID | 30416041
(Publication Type: Journal Article)
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Copyright | Copyright © 2018 IAP and EPC. Published by Elsevier B.V. All rights reserved. |
Chemical References |
- CLDN7 protein, human
- Claudins
- RNA, Small Interfering
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Topics |
- Animals
- Cell Line, Tumor
- Cell Proliferation
- Claudins
(genetics, metabolism)
- G1 Phase Cell Cycle Checkpoints
- Gene Expression Regulation, Neoplastic
- Gene Knockdown Techniques
- Humans
- Mice
- Neoplasms, Experimental
- Oligonucleotide Array Sequence Analysis
- Pancreatic Neoplasms
(drug therapy)
- RNA, Small Interfering
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