Down-regulation of miR-500 and miR-628 suppress non-small cell lung cancer proliferation, migration and invasion by targeting ING1.

Abstract

BACKGROUND:

MicroRNAs (miRNAs) have been consistently demonstrated to be involved in non-small cell lung cancer (NSCLC) as either tumor oncogenes or tumor suppressors. However, the detailed role of miR-500 and miR-628 in NSCLC remain poorly understood.

METHODS:

The expressions of miR-500 and miR-628 in NSCLC tissues and cell lines were measured by quantitative real-time PCR (qRT-PCR). Cells migration, invasion, proliferation, adhesion and apoptosis abilities were test to analyze the biological functions of miR-500 and miR-628 in NSCLC. A bioinformatic analysis was conducted to predict the target genes regulated by miR-500 and miR-628 using TargetScan (http://www.targetscan.org/mamm/). Luciferase reporter assay was employed to validate the direct targeting of ING1 by miR-500 and miR-628.

RESULTS:

In this study, miR-500 and miR-628 were up-regulated with NSCLC tissues. Furthermore, inhibition of miR-500 and miR-628 significantly suppressed NSCLC cells proliferation, migration, invasion and adhesion, and induced NSCLC cells apoptosis. Additionally, the result showed that ING1 functioned as the direct target for miR-500 and miR-628, which was a core tumor suppressor in regulating NSCLC progression. Over-expression of ING1 could dramatically inhibit NSCLC cells proliferation, migration and invasion, and promote cells apoptosis.

CONCLUSION:

These results brought new insights into the oncogenic role of miR-500 and miR-628 in NSCLC, indicating that miR-500 and miR-628 might be the novel biomarkers for the diagnosis and prognosis of NSCLC.

• Authors: Ming Jiang, Li-Yang Zhou, Nan Xu, Qing An
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 108 Pg. 1628-1639 (Dec 2018) ISSN: 1950-6007 [Electronic] France
• PMID: 30372865 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier Masson SAS. All rights reserved.

Chemical References

• ING1 protein, human
• Inhibitor of Growth Protein 1
• MIRN500 microRNA, human
• MIRN628 microRNA, human
• MicroRNAs

Topics

• A549 Cells
• Carcinoma, Non-Small-Cell Lung (metabolism, prevention & control)
• Cell Movement (physiology)
• Cell Proliferation (physiology)
• Down-Regulation (physiology)
• Gene Targeting (methods)
• Humans
• Inhibitor of Growth Protein 1 (antagonists & inhibitors, biosynthesis)
• Lung Neoplasms (metabolism, prevention & control)
• MicroRNAs (antagonists & inhibitors, metabolism)