Folate receptor-targeted liposomal nanocomplex for effective synergistic photothermal-chemotherapy of breast cancer in vivo.

Abstract

An effective nanoparticle-based drug delivery platform holds great promise for non-invasive cancer therapy. This study explores the breast tumor regression in vivo by synergistic photothermal-chemotherapy based on liposomal nanocomplex (folic acid-gold nanorods-anticancer drug-liposome). The proposed liposomal nanocomplex can enhance the tumor targeting by functionalizing folic acid (FA) molecules on the surface of liposome that encapsulates both gold nanorods (AuNRs) and the doxorubicin (DOX) to combine the photothermal therapy and the chemotherapy, respectively. Herein, 7-nm gold nanorods were fabricated and co-encapsulated with DOX into nanoliposomes functionalized with FA, with an average diameter of 154 nm, for active targeting to the cancer cells. The experimental results showed that the FA targeting liposomes had better cellular uptake than the non-targeting liposomes (AuNRs-DOX-LPs). Especially, upon 5 min exposure to near infrared (NIR) irradiation (808 nm) triggered DOX release could be achieved to 46.38% in 60 min at pH 5.5. In addition, in vitro cell proliferation assays indicated that, with synergistic photothermal-chemotherapy, the targeting liposomes could significantly enhance the toxicity towards the cancer cells with the IC50 value of 1.90 ± 0.12 μg mL-1. Furthermore, in vivo experiments on the breast tumor-bearing mice showed that the targeting liposomes could effectively inhibit the growth of the tumors using the combined strategy.

• Authors: Van Du Nguyen, Hyun-Ki Min, Chang-Sei Kim, Jiwon Han, Jong-Oh Park, Eunpyo Choi
• Journal: Colloids and surfaces. B, Biointerfaces (Colloids Surf B Biointerfaces) Vol. 173 Pg. 539-548 (Jan 01 2019) ISSN: 1873-4367 [Electronic] Netherlands
• PMID: 30343218 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier B.V. All rights reserved.

Chemical References

• Antibiotics, Antineoplastic
• Folate Receptors, GPI-Anchored
• Liposomes
• Gold
• Doxorubicin
• Folic Acid

Topics

• Animals
• Antibiotics, Antineoplastic (chemistry, metabolism, pharmacology)
• Cell Line, Tumor
• Combined Modality Therapy (methods)
• Doxorubicin (chemistry, metabolism, pharmacology)
• Drug Compounding (methods)
• Drug Liberation
• Female
• Folate Receptors, GPI-Anchored (metabolism)
• Folic Acid (chemistry, metabolism)
• Gold (chemistry)
• Hydrogen-Ion Concentration
• Infrared Rays
• Injections, Subcutaneous
• Liposomes (administration & dosage, chemistry)
• Low-Level Light Therapy (methods)
• Mammary Glands, Animal (metabolism, pathology)
• Mice
• Molecular Targeted Therapy (methods)
• Nanotubes (chemistry)
• Neoplasms (therapy)
• Protein Binding