Bladder cancer has become the most common malignant urinary
carcinoma. Studies have shown that significant
antioxidant and
bladder cancer-fighting properties of several
plant-based diets like Psidium guajava, ginger and amomum, are associated with their high
kaempferol content. In this paper, we evaluated the
antioxidant and anticancer activities of
kaempferol and its mechanism of induction to apoptosis on
bladder cancer cells. Our findings demonstrated that
kaempferol showed an obvious radical scavenging activity in erythrocytes damaged by
oxygen.
Kaempferol promoted
antioxidant enzymes, inhibited ROS generation and lipid peroxidation and finally prevented the occurrence of
hemolysis. Additionally,
kaempferol exhibited a strong inhibitory effect on
bladder cancer cells and high safety on normal bladder cells. At the molecular level,
kaempferol suppressed EJ
bladder cancer cell proliferation by inhibiting the function of phosphorylated AKT (p-AKT), CyclinD1, CDK4, Bid, Mcl-1 and Bcl-xL, and promoting p-BRCA1, p-ATM, p53, p21, p38, Bax and Bid expression, and finally triggering apoptosis and S phase arrest. We found that
Kaempferol exhibited strong
anti-oxidant activity on erythrocyte and inhibitory effects on the growth of cancerous bladder cells through inducing apoptosis and S phase arrest. These findings suggested that
kaempferol might be regarded as a bioactive
food ingredient to prevent oxidative damage and treat
bladder cancer.