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Tannic acid-inspired paclitaxel nanoparticles for enhanced anticancer effects in breast cancer cells.

Abstract
Paclitaxel (PTX) is a gold standard chemotherapeutic agent for breast, ovarian, pancreatic and non-small cell lung carcinoma. However, in clinical use PTX can have adverse side effects or inadequate pharmacodynamic parameters, limiting its use. Nanotechnology is often employed to reduce the therapeutic dosage required for effective therapy, while also minimizing the systemic side effects of chemotherapy drugs. However, there is no nanoformulation of paclitaxel with chemosensitization motifs built in. With this objective, we screened eleven pharmaceutical excipients to develop an alternative paclitaxel nanoformulation using a self-assembly method. Based on the screening results, we observed tannic acid possesses unique properties to produce a paclitaxel nanoparticle formulation, i.e., tannic acid-paclitaxel nanoparticles. This stable TAP nanoformulation, referred to as TAP nanoparticles (TAP NPs), showed a spherical shape of ~ 102 nm and negative zeta potential of ~ -8.85. The presence of PTX in TAP NPs was confirmed by Fourier Transform Infrared (FTIR) spectra, thermogravimetric analyzer (TGA), and X-ray diffraction (XRD). Encapsulation efficiency of PTX in TAP NPs was determined to be ≥96%. Intracellular drug uptake of plain drug PTX on breast cancer cells (MDA-MB-231) shows more or less constant drug levels in 2 to 6 h, suggesting drug efflux by the P-gp transporters, over TAP NPs, in which PTX uptake was more than 95.52 ± 11.01% in 6 h, as analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Various biological assays such as proliferation, clonogenic formation, invasion, and migration confirm superior anticancer effects of TAP NPs over plain PTX at all tested concentrations. P-gp expression, beta-tubulin stabilization, Western blot, and microarray analysis further confirm the improved therapeutic potential of TAP NPs. These results suggest that the TAP nanoformulation provides an important reference for developing a therapeutic nanoformulation affording pronounced, enhanced effects in breast cancer therapy.
AuthorsPallabita Chowdhury, Prashanth K B Nagesh, Elham Hatami, Santosh Wagh, Nirnoy Dan, Manish K Tripathi, Sheema Khan, Bilal B Hafeez, Bernd Meibohm, Subhash C Chauhan, Meena Jaggi, Murali M Yallapu
JournalJournal of colloid and interface science (J Colloid Interface Sci) Vol. 535 Pg. 133-148 (Feb 01 2019) ISSN: 1095-7103 [Electronic] United States
PMID30292104 (Publication Type: Journal Article)
CopyrightCopyright © 2018 Elsevier Inc. All rights reserved.
Chemical References
  • Antineoplastic Agents, Phytogenic
  • Tannins
  • Paclitaxel
Topics
  • Antineoplastic Agents, Phytogenic (chemistry, pharmacology)
  • Breast Neoplasms (drug therapy, metabolism, pathology)
  • Cell Movement (drug effects)
  • Cell Proliferation (drug effects)
  • Chromatography, Liquid
  • Drug Screening Assays, Antitumor
  • Female
  • Humans
  • MCF-7 Cells
  • Nanoparticles (chemistry)
  • Paclitaxel (chemistry, pharmacology)
  • Particle Size
  • Surface Properties
  • Tandem Mass Spectrometry
  • Tannins (chemistry)
  • Tumor Cells, Cultured

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