As expression level of allergic disease-sensitive genes are correlated with allergic symptom severity, suppression of these gene expressions could be good
therapeutics. We have demonstrated that PKCδ signaling and NFAT signaling, involve in
histamine H1 receptor (H1R) and
IL-9 gene expressions, respectively, are responsible for the pathogenesis of
allergic rhinitis. We explore
anti-allergic compounds that suppress these signaling pathways and found that wild grape (WG) contains such compounds. Here, we investigated the effect of WG hot water extract (WGE) on the signaling pathways for PKCδ-mediated H1R and NFAT-mediated
IL-9 gene expressions. WGE suppressed
histamine/PMA-induced H1R gene up-regulation in HeLa cells. Toluene-2,4-diisocyanate (TDI)-induced H1R
mRNA elevation in TDI-sensitized rats was also suppressed by WGE treatment. Treatment with WGE in combination with Awa-
tea, suppresses NFAT signaling-mediated
IL-9 gene, markedly alleviated nasal symptoms. Furthermore, WGE suppressed PMA-induced
IL-33 gene up-regulation in Swiss 3T3 cells. Data suggest that combination of WGE, suppresses PKCδ signaling with Awa-
tea, suppresses NFAT signaling would have distinct clinical and therapeutic advantages as a substitute for
anti-allergic drugs. In addition, as the expression level of
IL-33 mRNA was correlated with the blood eosinophils number in patients with
pollinosis, WG could alleviate eosinophilic
inflammation through the suppression of
IL-33 gene expression. J. Med. Invest. 65:242-250, August, 2018.