Abstract | Objective: To analyze the components of excretory-secretory protein(ESP) of Trichinella spiralis muscle larvae, and search for the anti- tumor protein components. Methods: The Trichinella spiralis muscle larvae were collected, and ESP was prepared. The ESP was separated in 15% SDS-PAGE. Proteins extracted from the protein bands were lysed with trypsin, and analyzed by LC-MS/MS. The identified proteins were classified by Gene Ontology(GO) according to cell component, molecular function, and biological processes. Results: Conclusion: The ESP of Trichinella spiralis muscle larvae has complex protein components, many with unknown identities. Six anti- tumor relevant proteins were determined from the 63 identified proteins.
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Authors | Jing-mei Luo, Lu-yang Cheng, Xiao-dong Guan, Dan Li, Li Yu, Luan-ying Du |
Journal | Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases
(Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi)
Vol. 34
Issue 1
Pg. 53-7
(Feb 2016)
ISSN: 1000-7423 [Print] China |
PMID | 30121063
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Helminth
- Helminth Proteins
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Topics |
- Animals
- Antigens, Helminth
- Chromatography, Liquid
- Electrophoresis, Polyacrylamide Gel
- Helminth Proteins
- Larva
- Mice
- Muscles
- Tandem Mass Spectrometry
- Trichinella spiralis
- Trichinellosis
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