Silkworm cocoon was recorded to cure
carbuncle in the Compendium of
Materia Medica. Previous studies have demonstrated that the supplemental
silk protein sericin exhibits anticancer activity. In the present study, we investigated the effects of
silk fibroin peptide (
SFP) extracted from silkworm cocoons against human
lung cancer cells in vitro and in vivo and its possible anticancer mechanisms.
SFP that we prepared had high content of
glycine (~ 30%) and showed a molecular weight of ~ 10 kDa. Intragastric administration of
SFP (30 g/kg/d) for 14 days did not affect the weights, vital signs, routine blood indices, and blood biochemical parameters in mice. MTT assay showed that
SFP dose-dependently inhibited the growth of human
lung cancer A549 and H460 cells in vitro with IC50 values of 9.921 and 9.083 mg/mL, respectively.
SFP also dose-dependently suppressed the clonogenic activity of the two cell lines. In
lung cancer H460 xenograft mice,
intraperitoneal injection of
SFP (200 or 500 mg/kg/d) for 40 days significantly suppressed the
tumor growth, but did not induce significant changes in the
body weight. We further examined the effects of
SFP on cell cycle and apoptosis in H460 cells using flow cytometry, which revealed that
SFP-induced cell cycle arrest at the S phase, and then promoted cell apoptosis. We demonstrated that
SFP (20-50 mg/mL) dose-dependently downregulates Bcl-2
protein expression and upregulates
Bax protein in H460 cells during cell apoptosis. The results suggest that
SFP should be studied further as a novel therapeutic agent for the treatment of
lung cancer.