Identification of N- and O-linked glycans recognized by AAL in saliva of patients with atrophic gastritis and gastric cancer.

Abstract

BACKGROUND AND AIM:

Gastric cancer (GC) is a common and fatal malignancy with a worldwide occurrence. There still lacks effective biomarkers for precisely evaluating GC. Saliva is a biological fluid with enormous diagnostic potentials which emerged many advantages. We aimed to discover the novel biomarkers for accurately distinguishing early GC based on saliva glycopatterns.

METHODS:

We used Aleuria Aurantia Lectin (AAL)-magnetic particle conjugates to isolate fucosylated glycoproteins in the pooled saliva of healthy volunteers (HV, n= 51) and patients with atrophic gastritis (AG, n= 51) or GC (n= 51), following to release the N- and O-linked glycans from the isolated proteins with PNGase F and NaClO, and further identified the released glycans by MALDI-TOF/TOF-MS, respectively.

RESULTS:

A total of 9/9, 8/11, and 9/9 fucosylated N-/O-linked glycans were annotated in the isolated salivary proteins from HV, AG, and GC, respectively. Among these, six fucosylated N-linked glycansand four O-linked glycans exhibited significantly increased expression levels in GC, while five fucosylated N-linked glycans and ten fucosylated O-linked glycans exhibited significantly decreased expression levels in GC. The proportion of fucosylated N-linked glycans was decreased in GC (41.66%) compared with AG (43.63%) and HV (52.57%), as well as the fucosylated O-linked glycans was apparently decreased in GC (19.58%) compared with AG (25.43%) and HV (55.54%).

CONCLUSIONS:

This study could provide pivotal information to distinguish among HV, AG, and GC, and facilitate the discovery of biomarkers for GC diagnosis based on precise alterations of N- and O-linked glycans in saliva.

• Authors: Jian Shu, Hanjie Yu, Haoqi Du, Jiaxu Zhang, Kun Zhang, Xiaojie Li, Hailong Xie, Zheng Li
• Journal: Cancer biomarkers : section A of Disease markers (Cancer Biomark) Vol. 22 Issue 4 Pg. 669-681 ( 2018) ISSN: 1875-8592 [Electronic] Netherlands
• PMID: 29865036 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• Lectins
• Polysaccharides
• lectin, Aleuria aurantia

Topics

• Adult
• Aged
• Biomarkers, Tumor (chemistry)
• Diagnosis, Differential
• Female
• Gastritis, Atrophic (diagnosis, metabolism, pathology)
• Glycosylation
• Healthy Volunteers
• Humans
• Lectins (chemistry)
• Male
• Middle Aged
• Polysaccharides (chemistry, isolation & purification)
• Saliva (chemistry)
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• Stomach Neoplasms (diagnosis, metabolism, pathology)