Abstract | BACKGROUND: RESULTS: 20 and 40 mg/kg osthole significantly alleviated collagen-induced arthritic symptoms based on histopathology and clinical arthritis scores, and improved erosion using HE staining. 20 and 40 mg/kg osthole decreased the level of IL-1β, TNF-α and IL-6 in rats and ameliorated oxidative stress in serum evaluated using ELISA kits. In addition, treatment with 50 and 100 μM osthole for 48 h inhibited 10 ng/ml IL-1β-stimulated proliferation and migration of SW982, and significantly inhibited the expression of matrix metalloproteinases, such as MMP-1, MMP-3 and MMP-13, as detected by western blot. 50 and 100 μM osthole also blocked the generation of IL-6 and TNF-α in IL-1β-stimulated SW982 cells. The NF-κB and MAPK pathways were also inhibited by osthole in IL-1β-treated SW982 cells. CONCLUSION: These results collectively demonstrated that osthole improves collagen-induced arthritis in a rat model and IL-1β-treated SW982 cells through inhibiting inflammation and cellular stress in vivo and in vitro, and osthole might be a promising therapeutic agent for RA.
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Authors | Renguo Xu, Zhen Liu, Jiande Hou, Tao Huang, Ming Yang |
Journal | Cellular & molecular biology letters
(Cell Mol Biol Lett)
Vol. 23
Pg. 19
( 2018)
ISSN: 1689-1392 [Electronic] England |
PMID | 29743895
(Publication Type: Journal Article)
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Chemical References |
- Anti-Inflammatory Agents, Non-Steroidal
- Coumarins
- Interleukin-1beta
- Interleukin-6
- Tumor Necrosis Factor-alpha
- osthol
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Topics |
- Animals
- Anti-Inflammatory Agents, Non-Steroidal
(administration & dosage, therapeutic use)
- Arthritis, Experimental
(chemically induced, drug therapy)
- Cell Movement
(drug effects)
- Cell Proliferation
(drug effects)
- Coumarins
(administration & dosage, pharmacology, therapeutic use)
- Hindlimb
(pathology)
- Humans
- Inflammation
(chemically induced, drug therapy)
- Interleukin-1beta
(blood)
- Interleukin-6
(blood)
- Male
- Oxidative Stress
(drug effects)
- Rats
- Rats, Sprague-Dawley
- Synoviocytes
(drug effects)
- Tumor Necrosis Factor-alpha
(blood)
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