miR-185 Inhibits the Proliferation and Invasion of Non-Small Cell Lung Cancer by Targeting KLF7.

Abstract

MicroRNAs (miRNAs) are short endogenous noncoding RNAs that frequently play vital roles in many cancer types. Herein we demonstrated that miR-185 was remarkably downregulated in NSCLC tissues compared with adjacent normal tissues. A lower level of miR-185 was associated with lymph node metastasis. Functional assays showed that upregulation of miR-185 inhibited the proliferation, colony formation, and invasion capacities of NSCLC cells in vitro. Furthermore, we found that miR-185 suppressed the epithelial-mesenchymal transition (EMT) process. Bioinformatics analysis and luciferase reporter gene assays revealed that Kruppel-like factor 7 (KLF7) was the target of miR-185. Overexpression of miR-185 reduced the expression of KLF7 in NSCLC cells. Upregulation of KLF7 partly neutralized the inhibitory effects of miR-185 on the proliferation and invasion of NSCLC. Additionally, we confirmed that miR-185 suppressed the tumor growth of NSCLC A549 cells in vivo. Taken together, these results demonstrate that miR-185 acts as a suppressor by targeting KLF7 in NSCLC.

• Authors: Lili Zhao, Yao Zhang, Jiaoxia Liu, Wei Yin, Dan Jin, Dandan Wang, Wei Zhang
• Journal: Oncology research (Oncol Res) Vol. 27 Issue 9 Pg. 1015-1023 (Sep 23 2019) ISSN: 1555-3906 [Electronic] United States
• PMID: 29716672 (Publication Type: Journal Article)

Chemical References

• KLF7 protein, human
• Kruppel-Like Transcription Factors
• MIRN185 microRNA, human
• MicroRNAs

Topics

• A549 Cells
• Animals
• Carcinoma, Non-Small-Cell Lung (genetics, metabolism, pathology)
• Cell Line, Tumor
• Cell Movement (physiology)
• Cell Proliferation (physiology)
• Down-Regulation
• Heterografts
• Humans
• Kruppel-Like Transcription Factors (genetics, metabolism)
• Lung Neoplasms (genetics, metabolism, pathology)
• Mice
• MicroRNAs (metabolism)
• Neoplasm Metastasis