Tween-20 transiently changes the surface morphology of PK-15 cells and improves PCV2 infection.

Abstract

BACKGROUND:

Low concentrations of nonionic surfactants can change the physical properties of cell membranes, and thus and in turn increase drug permeability. Porcine circovirus 2 (PCV2) is an extremely slow-growing virus, and PCV2 infection of PK-15 cells yields very low viral titers. The present study investigates the effect of various nonionic surfactants, namely, Tween-20, Tween-28, Tween-40, Tween-80, Brij-30, Brij-35, NP-40, and Triton X-100 on PCV2 infection and yield in PK-15 cells.

RESULT:

Significantly increased PCV2 infection was observed in cells treated with Tween-20 compared to those treated with Tween-28, Tween-40, Brij-30, Brij-35, NP-40, and Triton X-100 (p < 0.01). Furthermore, 24 h incubation with 0.03% Tween-20 has shown to induce significant cellular morphologic changes (cell membrane underwent slight intumescence and bulged into a balloon, and the number of microvilli decreased), as well as to increase caspase-3 activity and to decrease cell viability in PCV2-infected PK-15 cells cmpared to control group; all these changes were restored to normal after Tween-20 has been washed out from the plate.

CONCLUSION:

Our data demonstrate that Tween-20 transiently changes the surface morphology of PK-15 cells and improves PCV2 infection. The findings of the present study may be utilized in the development of a PCV2 vaccine.

• Authors: Tao Hua, Xuehua Zhang, Bo Tang, Chen Chang, Guoyang Liu, Lei Feng, Yang Yu, Daohua Zhang, Jibo Hou
• Journal: BMC veterinary research (BMC Vet Res) Vol. 14 Issue 1 Pg. 138 (Apr 24 2018) ISSN: 1746-6148 [Electronic] England
• PMID: 29699558 (Publication Type: Journal Article)

Chemical References

• Polysorbates
• Surface-Active Agents
• Caspase 3

Topics

• Animals
• Caspase 3 (metabolism)
• Cell Line
• Cell Survival (drug effects)
• Circoviridae Infections (veterinary, virology)
• Circovirus (metabolism, physiology)
• Kidney (cytology, virology)
• Microscopy, Electrochemical, Scanning (veterinary)
• Polysorbates (pharmacology)
• Surface-Active Agents (pharmacology)
• Swine
• Virus Replication (drug effects)