Pharmacokinetics of Caffeine in the Lens Capsule/Epithelium After Peroral Intake: A Pilot Randomized Controlled Study.

Abstract

Purpose:

To determine the pharmacokinetics of perorally administered caffeine, a widely consumed and potent dietary antioxidant, in the anterior lens capsule and lens epithelial cells, a crucial cell monolayer for cataract development.

Methods:

Bilateral cataract patients were scheduled for cataract surgery with a caffeine abstinence of 1 week before surgery of each eye. At the day of surgery of the second eye patients were administered no drink (0-mg group) or coffee with 60-, 120-, or 180-mg caffeine. After capsulorhexis the lens capsule including lens epithelial cells was transferred to a test tube for analysis of caffeine concentration by gas chromatography-mass spectrometry (GC-MS/MS).

Results:

Coffee consumption significantly (P < 0.05) increased caffeine levels of the lens capsule/epithelium in the 60-, 120-, and 180-mg group. Caffeine concentrations (caffeine ng/lens capsule/epithelium) measured as difference between 1st and 2nd eye were -0.52 ± 1.16 (0-mg group, n = 7), 1.88 ± 2.02 (60-mg group, n = 8), 2.09 ± 0.67 (120-mg group, n = 9), and 3.68 ± 1.86 (180-mg group, n = 9). The increase constant of caffeine in a linear regression model was estimated as a 95% CI 0.02 ± 0.0046 (degrees of freedom; 25; r = 0.85).

Conclusions:

Peroral intake of coffee significantly increased caffeine concentrations in the lens capsule and lens epithelial cells in a dose-dependent manner. This information is important for further investigations on preventing cataract.

• Authors: Martin Kronschläger, Thomas Stimpfl, Manuel Ruiß, Nino Hirnschall, Christoph Leisser, Oliver Findl
• Journal: Investigative ophthalmology & visual science (Invest Ophthalmol Vis Sci) Vol. 59 Issue 5 Pg. 1855-1860 (04 01 2018) ISSN: 1552-5783 [Electronic] United States
• PMID: 29677345 (Publication Type: Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)

Chemical References

• Central Nervous System Stimulants
• Coffee
• Caffeine

Topics

• Administration, Oral
• Aged
• Anterior Capsule of the Lens (metabolism)
• Caffeine (pharmacokinetics)
• Cataract (complications)
• Cataract Extraction
• Central Nervous System Stimulants (pharmacokinetics)
• Coffee
• Epithelial Cells (metabolism)
• Female
• Gas Chromatography-Mass Spectrometry
• Humans
• Lens, Crystalline (cytology)
• Male
• Pilot Projects
• Tandem Mass Spectrometry
• Tissue Distribution