Long non-coding RNA BANCR indicates poor prognosis for breast cancer and promotes cell proliferation and invasion.

Abstract

OBJECTIVE:

We investigated the expression of human long non-coding ribonucleic acid (lncRNA), BRAF-activated non-coding RNA (BANCR) in breast cancer tissues and its effects on the in vitro proliferation, apoptosis, invasion and metastasis of breast cancer cells; also, we investigated its possible mechanism.

PATIENTS AND METHODS:

The expressions of BANCR in 65 pairs of breast cancer tissues and para-carcinoma normal breast tissues were detected by Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR). The expressions of BANCR in normal breast epithelial cells (MCF10A) and breast cancer cells (MCF-7, MDA-MB-231, SKBR3 and BT-20) were further detected. The knockdown efficiency of BANCR small interfering RNA (siRNA) in MCF-7 cells was detected by qRT-PCR. The effects of BANCR knockdown on proliferation, apoptosis, invasion and metastasis capacities of MCF-7 cells were explored via methyl thiazolyl tetrazolium (MTT) proliferation assay, cell colony formation assay, fluorescence-activated cell sorting (FACS) and transwell migration assay. Western blotting was used to detect the changes in expressions of apoptosis-related proteins, epithelial-mesenchymal transition (EMT)-related proteins and matrix metalloproteinases (MMPs) after knockdown of BANCR.

RESULTS:

The expression level of lncRNA BANCR in breast cancer tissues was significantly higher than that in para-carcinoma normal tissues. The prognosis of patients in low-expression BANCR group was significantly superior to that of patients in high-expression BANCR group. After BANCR knockdown in breast cancer MCF-7 cells, the cell proliferation and colony formation capacities were significantly inhibited. Further mechanism research showed that inhibiting BANCR could promote the apoptosis of MCF-7 cells. Results of Western blotting revealed that the expressions of B-cell lymphoma 2 associated X protein (BAX), cleaved-Caspase-3 and cleaved-poly adenosine diphosphate-ribose polymerase (PARP) in knockdown group were significantly up-regulated compared with those in control group. Both wound-healing assay and transwell migration assay showed that the down-regulation of lncRNA BANCR could inhibit the invasion and metastasis capacities of MCF-7 cells, whose mechanism was related to the inhibition of EMT process and down-regulation of MMP expressions in cells.

CONCLUSIONS:

LncRNA BANCR is highly expressed in breast cancer, which is significantly correlated with the prognosis of patients; moreover, it can promote the growth, invasion and metastasis of ovarian cancer cells. The down-regulation of BANCR can inhibit the proliferation, invasion and metastasis capacities of MCF-7 cells.

• Authors: K-X Lou, Z-H Li, P Wang, Z Liu, Y Chen, X-L Wang, H-X Cui
• Journal: European review for medical and pharmacological sciences (Eur Rev Med Pharmacol Sci) Vol. 22 Issue 5 Pg. 1358-1365 (03 2018) ISSN: 2284-0729 [Electronic] Italy
• PMID: 29565494 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• BANCR long non-coding RNA, human
• RNA, Long Noncoding
• RNA, Small Interfering
• bcl-2-Associated X Protein
• Poly(ADP-ribose) Polymerases
• Proto-Oncogene Proteins B-raf
• Matrix Metalloproteinase 2

Topics

• Apoptosis
• Breast Neoplasms (metabolism, mortality, pathology)
• Cell Line, Tumor
• Cell Movement
• Cell Proliferation
• Epithelial-Mesenchymal Transition
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Kaplan-Meier Estimate
• Matrix Metalloproteinase 2 (metabolism)
• Middle Aged
• Poly(ADP-ribose) Polymerases (metabolism)
• Prognosis
• Proto-Oncogene Proteins B-raf (genetics)
• RNA Interference
• RNA, Long Noncoding (antagonists & inhibitors, genetics, metabolism)
• RNA, Small Interfering (metabolism)
• bcl-2-Associated X Protein (metabolism)