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PAF-Myc-Controlled Cell Stemness Is Required for Intestinal Regeneration and Tumorigenesis.

Abstract
The underlying mechanisms of how self-renewing cells are controlled in regenerating tissues and cancer remain ambiguous. PCNA-associated factor (PAF) modulates DNA repair via PCNA. Also, PAF hyperactivates Wnt/β-catenin signaling independently of PCNA interaction. We found that PAF is expressed in intestinal stem and progenitor cells (ISCs and IPCs) and markedly upregulated during intestinal regeneration and tumorigenesis. Whereas PAF is dispensable for intestinal homeostasis, upon radiation injury, genetic ablation of PAF impairs intestinal regeneration along with the severe loss of ISCs and Myc expression. Mechanistically, PAF conditionally occupies and transactivates the c-Myc promoter, which induces the expansion of ISCs/IPCs during intestinal regeneration. In mouse models, PAF knockout inhibits Apc inactivation-driven intestinal tumorigenesis with reduced tumor cell stemness and suppressed Wnt/β-catenin signaling activity, supported by transcriptome profiling. Collectively, our results unveil that the PAF-Myc signaling axis is indispensable for intestinal regeneration and tumorigenesis by positively regulating self-renewing cells.
AuthorsMoon Jong Kim, Bo Xia, Han Na Suh, Sung Ho Lee, Sohee Jun, Esther M Lien, Jie Zhang, Kaifu Chen, Jae-Il Park
JournalDevelopmental cell (Dev Cell) Vol. 44 Issue 5 Pg. 582-596.e4 (03 12 2018) ISSN: 1878-1551 [Electronic] United States
PMID29533773 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2018 Elsevier Inc. All rights reserved.
Chemical References
  • Carrier Proteins
  • Myc protein, mouse
  • PAF protein, mouse
  • Proto-Oncogene Proteins c-myc
Topics
  • Animals
  • Carcinogenesis (genetics, metabolism, pathology)
  • Carrier Proteins (physiology)
  • Cell Proliferation
  • Female
  • Gene Expression Profiling
  • Homeostasis
  • Intestinal Mucosa (metabolism)
  • Intestines (pathology)
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Neoplastic Stem Cells (metabolism, pathology)
  • Pluripotent Stem Cells (metabolism, pathology)
  • Proto-Oncogene Proteins c-myc (physiology)
  • Regeneration (physiology)
  • Wnt Signaling Pathway

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