The left
middle cerebral artery occlusion (MCAO) model was made by inserting the
nylon thread plug into the internal carotid artery. The behavioral score,
cerebral infarction area, brain water content,
ethidium bromide (EB) spillover, coagulation four indices,
occludin and MMP-9 expression in brain tissues were detected after 14 days of administration, to investigate whether the protective effect of ginkgo
diterpene lactone meglumine injection (GDLMI) which had obvious protective effect on cerebral ischemic injury in the previous experiment was related to reducing the permeability of the blood-brain barrier (BBB) and reducing the risk of
bleeding, and to explore its possible mechanism of action. The results showed that GDLMI could effectively alleviate the behavioral changes caused by MCAO at 24 h, reduce the behavioral score, improve the
edema of brain tissue, reduce the EB overflow rate, reduce the
bleeding tendency caused by long-term administration, significantly reduce the occlusion deficiency in ischemic brain tissue of model rats, and down-regulate MMP-9 expression. The above results indicate that GDLMI has obvious effect on
cerebral ischemia, and the
therapeutic effect of GDLMI may mainly depend on lowering the permeability of blood-brain barrier to improve
brain edema.