Abstract |
Exposure to environmental endocrine disruptors (EEDs) contributes to the pathogenesis of many metabolic disorders. Here, we have analyzed the effect of the EED- nonylphenol (NP) on the promotion of non-alcoholic fatty liver disease ( NAFLD) in rats fed high sucrose-high fat diet (HSHFD). Fifty Sprague-Dawley rats were divided into five groups: controls fed a normal diet (C-ND); HSHFD-fed controls (C-HSHFD); and rats fed a HSHFD combined with NP at doses of 0.02 μg/kg/day (NP-L-HSHFD), 0.2 μg/kg/day (NP-M-HSHFD), and 2 μg/kg/day (NP-H-HSHFD). Subchronic exposure to NP coupled with HSHFD increased daily water and food intake (p < 0.05), hepatic echogenicity and oblique liver diameter (p < 0.05), and plasma levels of alanine aminotransferase, aspartate aminotransferase, total cholesterol, triglycerides, and low density lipoprotein cholesterol (p < 0.05). Combined exposure to NP and HSHFD induced macrovesicular steatosis with dilation and congestion of the central vein, liver inflammatory cell infiltration, and expression of genes regulating lipid metabolism, SREBP-1C, FAS, and Ucp2. These results demonstrate that NP aggravates NAFLD in HSHFD-treated rats by up-regulating lipogenic genes, and that HSHFD increases the toxic effects of NP. Thus subchronic NP exposure may lead to NAFLD, especially when combined with a high- sucrose/high-fat diet.
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Authors | Jie Yu, Xuesong Yang, Xuefeng Yang, Mengxue Yang, Pan Wang, Yu Yang, Jing Yang, Wenmei Li, Jie Xu |
Journal | Scientific reports
(Sci Rep)
Vol. 8
Issue 1
Pg. 3232
(02 19 2018)
ISSN: 2045-2322 [Electronic] England |
PMID | 29459774
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Endocrine Disruptors
- Environmental Pollutants
- Fats
- Lipoproteins, LDL
- Phenols
- Triglycerides
- Sucrose
- nonylphenol
- Cholesterol
- Aspartate Aminotransferases
- Alanine Transaminase
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Topics |
- Alanine Transaminase
(blood)
- Animals
- Aspartate Aminotransferases
(blood)
- Blood Chemical Analysis
- Cholesterol
(blood)
- Diet
(methods)
- Endocrine Disruptors
(toxicity)
- Environmental Pollutants
(toxicity)
- Fats
(administration & dosage)
- Fatty Liver
(pathology)
- Lipoproteins, LDL
(blood)
- Liver
(pathology)
- Non-alcoholic Fatty Liver Disease
(pathology)
- Phenols
(toxicity)
- Rats, Sprague-Dawley
- Sucrose
(administration & dosage)
- Triglycerides
(blood)
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