Abstract |
Mesenchymal stromal cells (MSCs) are multipotent stem cells with immunosuppressive and trophic support functions. While MSCs from different sources frequently display a similar appearance in culture, they often show differences in their surface marker and gene expression profiles. Although bone marrow is considered the "gold standard" tissue to isolate classical MSCs (BM-MSC), MSC-like cells are currently also derived from more easily accessible extra-embryonic tissues such as the umbilical cord. In this study, we defined the best way to isolate MSCs from the Wharton's jelly of the human umbilical cord (WJ-MSC) and assessed the mesenchymal and immunological phenotype of BM-MSC and WJ-MSC. Moreover, the gene expression profile of established WJ-MSC cultures was compared to two different bone marrow-derived stem cell populations (BM-MSC and multipotent adult progenitor cells or MAPC®). We observed that explant culturing of Wharton's jelly matrix is superior to collagenase tissue digestion for obtaining mesenchymal-like cells, with explant isolated cells displaying increased expansion potential. While being phenotypically similar to adult MSCs, WJ-MSC show a different gene expression profile. Gene ontology analysis revealed that genes associated with cell adhesion, proliferation, and immune system functioning are enriched in WJ-MSC. In vivo transplantation confirms their immune modulatory effect on T cells, similar to BM-MSC and MAPC. Furthermore, WJ-MSC intrinsically overexpress genes involved in neurotrophic support and their secretome induces neuronal maturation of SH-SY5Y neuroblastoma cells to a greater extent than BM-MSC. This signature makes WJ-MSC an attractive candidate for cell-based therapy in neurodegenerative and immune-mediated central nervous system disorders such as multiple sclerosis, Parkinson's disease, or amyotrophic lateral sclerosis.
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Authors | Raf Donders, Jeroen F J Bogie, Stylianos Ravanidis, Pascal Gervois, Marjan Vanheusden, Raphaël Marée, Marie Schrynemackers, Hubert J M Smeets, Jef Pinxteren, Kristel Gijbels, Sara Walbers, Robert W Mays, Robert Deans, Ludo Van Den Bosch, Piet Stinissen, Ivo Lambrichts, Wilfried Gyselaers, Niels Hellings |
Journal | Stem cells and development
(Stem Cells Dev)
Vol. 27
Issue 2
Pg. 65-84
(01 15 2018)
ISSN: 1557-8534 [Electronic] United States |
PMID | 29267140
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Bone Marrow Cells
(cytology, immunology)
- Cell Adhesion
(immunology)
- Cell Line, Tumor
(cytology, immunology)
- Cell Proliferation
(physiology)
- Gene Expression Profiling
- Gene Expression Regulation
(immunology)
- Gene Ontology
- Humans
- Immunomodulation
- Mesenchymal Stem Cells
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