Abstract |
Fibrosarcomas are highly aggressive malignant tumors. It is urgently needed to explore targeted drugs and modalities for more effective therapy. Matrix metalloproteinases ( MMPs) play important roles in tumor progression and metastasis, while several MMPs are highly expressed in fibrosarcomas. In addition, tissue inhibitor of metalloproteinase 2 (TIMP2) displays specific interaction with MMPs. Therefore, TIMP2 may play an active role in the development of fibrosarcoma-targeting agents. In the current study, a TIMP2-based recombinant protein LT and its enediyne-integrated analog LTE were prepared; furthermore, the fibrosarcoma-binding intensity and antitumor activity were investigated. As shown, intense and selective binding capability of the protein LT to human fibrosarcoma specimens was confirmed by tissue microarray. Moreover, LTE, the enediyne-integrated analog of LT, exerted highly potent cytotoxicity to fibrosarcoma HT1080 cells, induced apoptosis, and caused G2/M arrest. LTE at 0.1 nM markedly suppressed the migration and invasion of HT1080 cells. LTE at tolerated dose of 0.6 mg/kg inhibited the tumor growth of fibrosarcoma xenograft in athymic mice. The study provides evidence that the TIMP2-based reconstituted analog LTE may be useful as a targeted drug for fibrosarcome therapy.
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Authors | Jian Xu, Yue Du, Wen-Juan Liu, Liang Li, Yi Li, Xiao-Fei Wang, Hong-Fei Yi, Chuan-Kun Shan, Gui-Min Xia, Xiu-Jun Liu, Yong-Su Zhen |
Journal | Drug delivery
(Drug Deliv)
Vol. 25
Issue 1
Pg. 102-111
(Nov 2018)
ISSN: 1521-0464 [Electronic] England |
PMID | 29250984
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- Tissue Inhibitor of Metalloproteinase-2
- Matrix Metalloproteinases
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Topics |
- A549 Cells
- Animals
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Cell Cycle Checkpoints
(drug effects)
- Cell Line, Tumor
- Cell Movement
(drug effects)
- Cell Proliferation
(drug effects)
- Fibrosarcoma
(drug therapy, metabolism)
- G2 Phase Cell Cycle Checkpoints
(drug effects)
- Humans
- Matrix Metalloproteinases
(metabolism)
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Tissue Inhibitor of Metalloproteinase-2
(metabolism)
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