Abstract | BACKGROUND: METHODS: IANX was performed and the mechanical head-withdrawal threshold (MHWT) in the whisker pad skin ipsilateral to IANX was measured for 15 days. Expression of Iba1 in the TG was examined on day 3 after IANX, and the MHWT in the whisker pad skin ipsilateral to IANX was measured following successive intra- ganglion administration of the macrophage depletion agent liposomal clodronate Clophosome-A (LCCA). TNFα expression in the TG and the MHWT in the whisker pad skin ipsilateral to IANX following successive intra- ganglion administration of the TNFα blocker etanercept were measured on day 3 after IANX, and tumor necrosis factor receptor-1 ( TNFR1) immunoreactive (IR) cells in the TG were analyzed immunohistochemically on day 3. RESULTS: The MHWT in the whisker pad skin was significantly decreased for 15 days, and the number of Iba1-IR cells was significantly increased in the TG on day 3 after IANX. Successive intra- ganglion administration of the macrophage depletion agent LCCA significantly reduced the increased number of Iba1-IR cells in the TG and reversed the IANX-induced decrease in MHWT in the whisker pad skin. TNFα expression was increased in the TG on day 3 after IANX and was reduced following successive intra- ganglion administration of the TNFα inhibitor etanercept. The decreased MHWT was also recovered by etanercept administration, and TNFR1-IR cells in the TG were increased on day 3 following IANX. CONCLUSIONS: These findings suggest that signaling cascades resulting from the production of TNFα by infiltrated macrophages in the TG contributes to the development of ectopic mechanical allodynia in whisker pad skin following IANX.
|
Authors | Dulguun Batbold, Masamichi Shinoda, Kuniya Honda, Akihiko Furukawa, Momoko Koizumi, Ryuta Akasaka, Satoshi Yamaguchi, Koichi Iwata |
Journal | Journal of neuroinflammation
(J Neuroinflammation)
Vol. 14
Issue 1
Pg. 249
(Dec 16 2017)
ISSN: 1742-2094 [Electronic] England |
PMID | 29246259
(Publication Type: Journal Article)
|
Chemical References |
- Tumor Necrosis Factor-alpha
|
Topics |
- Animals
- Facial Pain
(etiology, immunology)
- Hyperalgesia
(immunology)
- Macrophages
(immunology)
- Male
- Mandibular Nerve
- Neuralgia
(immunology)
- Physical Stimulation
- Rats
- Rats, Sprague-Dawley
- Trigeminal Ganglion
(immunology)
- Trigeminal Nerve Injuries
(complications, immunology)
- Tumor Necrosis Factor-alpha
(biosynthesis)
|