Abstract |
Sepsis-associated acute respiratory distress syndrome (ARDS) is characterized by neutrophilic inflammation and poor survival. Since neutrophil myeloperoxidase (MPO) activity leads to increased plasma 2-chlorofatty acid (2-ClFA) levels, we hypothesized that plasma concentrations of 2-ClFAs would associate with ARDS and mortality in subjects with sepsis. In sequential consenting patients with sepsis, free 2-ClFA levels were significantly associated with ARDS, and with 30-day mortality, for each log increase in free 2-chlorostearic acid. Plasma MPO was not associated with either ARDS or 30-day mortality but was correlated with 2-ClFA levels. Addition of plasma 2-ClFA levels to the APACHE III score improved prediction for ARDS. Plasma 2-ClFA levels correlated with plasma levels of angiopoietin-2, E selectin, and soluble thrombomodulin. Endothelial cells treated with 2-ClFA responded with increased adhesion molecule surface expression, increased angiopoietin-2 release, and dose-dependent endothelial permeability. Our results suggest that 2-ClFAs derived from neutrophil MPO-catalyzed oxidation contribute to pulmonary endothelial injury and have prognostic utility in sepsis-associated ARDS.
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Authors | Nuala J Meyer, John P Reilly, Rui Feng, Jason D Christie, Stanley L Hazen, Carolyn J Albert, Jacob D Franke, Celine L Hartman, Jane McHowat, David A Ford |
Journal | JCI insight
(JCI Insight)
Vol. 2
Issue 23
(12 07 2017)
ISSN: 2379-3708 [Electronic] United States |
PMID | 29212955
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Biomarkers
- Fatty Acids
- Hydrocarbons, Chlorinated
- MPO protein, human
- Peroxidase
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Topics |
- APACHE
- Aged
- Biomarkers
(blood)
- Fatty Acids
(blood)
- Female
- Humans
- Hydrocarbons, Chlorinated
(blood)
- Male
- Middle Aged
- Peroxidase
(blood)
- Prognosis
- Respiratory Distress Syndrome
(blood, etiology, mortality)
- Sepsis
(blood, complications, mortality)
- United States
(epidemiology)
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