E. fischeriana Root Compound Dpo Activates Antiviral Innate Immunity.

Abstract

E. fischeriana has long been used as a traditional Chinese medicine. Recent studies reported that some compounds of E. fischeriana exhibited antimicrobial and immune enhance activity. Innate immune system is essential for the immune surveillance of inner and outer threats, initial host defense responses and immune modulation. The role of natural drug compounds, including E. fischeriana, in innate immune regulation is largely unknown. Here we demonstrated that E. fischeriana compound Dpo is involved in antiviral signaling. The genome wide RNA-seq analysis revealed that the induction of ISGs by viral infection could be synergized by Dpo. Consistently, Dpo enhanced the antiviral immune responses and protected the mice from death during viral infection. Dpo however was not able to rescue STING deficient mice lethality caused by HSV-1 infection. The enhancement of ISG15 by Dpo was also impaired in STING, IRF3, IRF7, or ELF4 deficient cells, demonstrating that Dpo activates innate immune responses in a STING/IRFs/ELF4 dependent way. The STING/IRFs/ELF4 axis is therefore important for Dpo induced ISGs expression, and can be used by host to counteract infection.

• Authors: Jingxuan Chen, Hongqiang Du, Shuang Cui, Tong Liu, Guang Yang, Huaping Sun, Weiwei Tao, Baoping Jiang, Li Yu, Fuping You
• Journal: Frontiers in cellular and infection microbiology (Front Cell Infect Microbiol) Vol. 7 Pg. 456 ( 2017) ISSN: 2235-2988 [Electronic] Switzerland
• PMID: 29124042 (Publication Type: Journal Article)

Chemical References

• Antiviral Agents
• Cytokines
• DNA-Binding Proteins
• Elf4 protein, mouse
• G1p2 protein, mouse
• Interferon Regulatory Factor-3
• Interferon Regulatory Factor-7
• Interferon Type I
• Irf3 protein, mouse
• Irf7 protein, mouse
• Membrane Proteins
• Plant Extracts
• RNA, Messenger
• Sting1 protein, mouse
• Transcription Factors
• Ubiquitins

Topics

• Animals
• Antiviral Agents (pharmacology)
• Cytokines (metabolism)
• DNA-Binding Proteins (metabolism)
• Euphorbia (chemistry)
• Gene Expression Regulation (drug effects)
• Herpes Simplex (drug therapy)
• Herpesvirus 1, Human (drug effects)
• Immunity, Innate
• Interferon Regulatory Factor-3 (metabolism)
• Interferon Regulatory Factor-7 (metabolism)
• Interferon Type I (drug effects)
• Macrophages (drug effects, virology)
• Medicine, Chinese Traditional
• Membrane Proteins (metabolism)
• Mice
• Plant Extracts (chemistry, pharmacology)
• Plant Roots (chemistry)
• RNA, Messenger (metabolism)
• Signal Transduction
• Transcription Factors (metabolism)
• Ubiquitins (metabolism)
• Viral Load