Study of bone metabolism and angiogenesis in patients undergoing high-dose chemotherapy/autologous hematopoietic stem cell transplantation.

Abstract

OBJECTIVES:

As the interaction between hematopoietic stem cells (HSCs) and endosteal and endothelial niches in HSCs homing is essential, we aimed to study bone turnover and angiogenesis in 29 patients with lymphoma/multiple myeloma undergoing hematopoietic stem cell transplantation (HSCT).

METHODS:

Serum samples were collected before high-dose chemotherapy (HDT), at the end of HDT, after HSC infusion, at the nadir of myelotoxicity, and at engraftment. Bone metabolism (CTX, TRACP-5b, bALP, OC, DKK1, RANKL, OPG), and angiogenesis (Ang1, Ang2) markers were measured. These markers were also measured in 21 control patients before and after conventional chemotherapy.

RESULTS AND CONCLUSIONS:

Bone resorption declined during HSCT (decrease in TRACP-5b [P < .001] and CTX [P = .006]). Bone formation declined as well (decrease in bALP and OC [P < .001 for both]). RANKL/OPG ratio, an indicator of osteoclastic activation, did not change significantly (P = .5). Ang1/Ang2 ratio, a vessel equilibrium marker, decreased significantly (P < .001) suggesting endothelial destabilization. The changes observed in the control group were similar except of bALP and RANKL/OPG ratio. Moreover, Ang1/Ang2 ratio on the day after HSC infusion strongly correlated with time to neutrophil and platelet engraftment (P < .001 for both). Conclusively, bone turnover and vessel destabilization represent important events during HSCT probably reflecting the effect of chemotherapy.

• Authors: Georgios Boutsikas, Evangelos Terpos, Athanasios Papatheodorou, Pantelis Tsirkinidis, Panayiotis Tsirigotis, Anastasia Meletiou, Eleni Lalou, Vasileios Telonis, Anna Zannou, Alexander Kanellopoulos, Zacharoula Galani, Angeliki Stefanou, Panayiotis Tsaftaridis, Nora-Athina Viniou, Panayiotis Panayiotidis, Marie-Christine Kyrtsonis, John Meletis, Theodoros P Vassilakopoulos, Maria K Angelopoulou
• Journal: European journal of haematology (Eur J Haematol) Vol. 100 Issue 2 Pg. 131-139 (Feb 2018) ISSN: 1600-0609 [Electronic] England
• PMID: 29105864 (Publication Type: Journal Article)
• Copyright: © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Chemical References

• Biomarkers

Topics

• Adult
• Aged
• Antineoplastic Combined Chemotherapy Protocols (adverse effects, therapeutic use)
• Biomarkers
• Bone Remodeling
• Bone Resorption (metabolism, pathology)
• Bone and Bones (metabolism, pathology)
• Female
• Graft Survival
• Hematopoietic Stem Cell Transplantation (adverse effects)
• Hematopoietic Stem Cells (metabolism)
• Humans
• Lymphoma (complications, pathology, therapy)
• Male
• Middle Aged
• Multiple Myeloma (complications, pathology, therapy)
• Neovascularization, Pathologic
• Osteogenesis
• Transplantation, Autologous
• Young Adult