Abstract | PURPOSE: To develop and validate a method for measuring murine single-kidney glomerular filtration rate (GFR) using dynamic contrast-enhanced MRI (DCE-MRI). METHODS: This prospective study was approved by the Institutional Animal Care and Use Committee. A fast longitudinal relaxation time (T1 ) measurement method was implemented to capture gadolinium dynamics (1 s/scan), and a modified two-compartment model was developed to quantify GFR as well as renal perfusion using 16.4T MRI in mice 2 weeks after unilateral renal artery stenosis (RAS, n = 6) or sham (n = 8) surgeries. This approach was validated by comparing model-derived GFR and perfusion to those obtained by fluorescein isothiocyanante ( FITC)-inulin clearance and arterial spin labeling (ASL), respectively, using the Pearson's and Spearman's rank correlations and Bland-Altman analysis. RESULTS: The compartmental model provided a good fitting to measured gadolinium dynamics in both normal and RAS kidneys. The proposed DCE-MRI method offered assessment of single-kidney GFR and perfusion, comparable to the FITC-inulin clearance (Pearson's correlation coefficient r = 0.95 and Spearman's correlation coefficient ρ = 0.94, P < 0.0001, and mean difference -7.0 ± 11.0 μL/min) and ASL (r = 0.92 and ρ = 0.84, P < 0.0001, and mean difference 4.4 ± 66.1 mL/100 g/min) methods. CONCLUSION: The proposed DCE-MRI method may be useful for reliable noninvasive measurements of single-kidney GFR and perfusion in mice. Magn Reson Med 79:2935-2943, 2018. © 2017 International Society for Magnetic Resonance in Medicine.
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Authors | Kai Jiang, Hui Tang, Prasanna K Mishra, Slobodan I Macura, Lilach O Lerman |
Journal | Magnetic resonance in medicine
(Magn Reson Med)
Vol. 79
Issue 6
Pg. 2935-2943
(06 2018)
ISSN: 1522-2594 [Electronic] United States |
PMID | 29034514
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Copyright | © 2017 International Society for Magnetic Resonance in Medicine. |
Chemical References |
- Contrast Media
- Spin Labels
- Inulin
- Fluorescein-5-isothiocyanate
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Topics |
- Animals
- Arteries
(pathology)
- Body Weight
- Contrast Media
(chemistry)
- Fluorescein-5-isothiocyanate
(chemistry)
- Glomerular Filtration Rate
- Image Interpretation, Computer-Assisted
(methods)
- Image Processing, Computer-Assisted
- Inulin
(chemistry)
- Kidney
(diagnostic imaging)
- Kidney Function Tests
(methods)
- Magnetic Resonance Imaging
- Mice
- Perfusion
- Prospective Studies
- Reproducibility of Results
- Spin Labels
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