Abstract |
Metastatic breast cancer affects long-term survival and is a major cause of cancer death for women worldwide. The Metalloprotease- Disintegrin ADAM8 promotes breast cancer development and brain metastasis in a mouse breast cancer model. Here, abundant ADAM8 expression was detected in primary human breast tumors and associated brain metastases. To investigate the function of ADAM8 in metastasis, MB-231 breast cancer cells with ADAM8 knockdown (MB-231_shA8) and scramble control cells (MB-231_shCtrl) were analyzed for their capability to develop metastases. In vitro, formation of metastatic complexes in hanging drops is dependent on ADAM8 and blocked by ADAM8 inhibition. MB-231_shA8 in contrast to MB-231_shCtrl cells were impaired in transmigration through an endothelial and a reconstituted blood-brain barrier. Out of 23 MMP and 22 ADAM genes, only the MMP-9 gene was affected by ADAM8 knockdown in MB-231_shA8 cells. Following re-expression of wild-type ADAM8 in contrast to ADAM8 lacking the cytoplasmic domain in MB-231_shA8 cells caused increased levels of activated pERK1/2 and pCREB (S133) that were associated with elevated MMP-9 transcription. Application of ADAM8 and MMP-9 antibodies reduced transmigration of MB-231 cells suggesting that ADAM8 affects transmigration of breast cancer cells by MMP-9 regulation. ADAM8-dependent transmigration was confirmed in Hs578t cells overexpressing ADAM8. Moreover, transmigration of MB-231 and Hs578t cells was significantly reduced for cells treated with an antibody directed against P-selectin glycoprotein ligand (PSGL-1), a substrate of ADAM8. From these data we conclude that ADAM8 promotes early metastatic processes such as transendothelial migration by upregulation of MMP-9 and shedding of PSGL-1 from breast cancer cells.
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Authors | Catharina Conrad, Malena Götte, Uwe Schlomann, Marion Roessler, Axel Pagenstecher, Peter Anderson, Jane Preston, Jessica Pruessmeyer, Andreas Ludwig, Ran Li, Roger D Kamm, Rainer Ritz, Barbara Carl, Christopher Nimsky, Jörg W Bartsch |
Journal | International journal of cancer
(Int J Cancer)
Vol. 142
Issue 4
Pg. 779-791
(02 15 2018)
ISSN: 1097-0215 [Electronic] United States |
PMID | 28986926
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2017 UICC. |
Chemical References |
- Membrane Glycoproteins
- Membrane Proteins
- P-selectin ligand protein
- ADAM Proteins
- ADAM8 protein, human
- MMP9 protein, human
- Matrix Metalloproteinase 9
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Topics |
- ADAM Proteins
(biosynthesis, genetics)
- Adult
- Aged
- Aged, 80 and over
- Brain Neoplasms
(genetics, metabolism, secondary)
- Breast Neoplasms
(genetics, metabolism, pathology)
- Cell Line, Tumor
- Cell Movement
(physiology)
- Coculture Techniques
- Endothelial Cells
(pathology)
- Female
- Human Umbilical Vein Endothelial Cells
- Humans
- Matrix Metalloproteinase 9
(biosynthesis, genetics)
- Membrane Glycoproteins
(metabolism)
- Membrane Proteins
(biosynthesis, genetics)
- Middle Aged
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