Epigallocatechin-3-gallate promotes apoptosis and reversal of multidrug resistance in esophageal cancer cells.

Abstract

Evidence for demonstrating the role of the green tea component epigallocatechin-3-gallate (EGCG) in esophageal squamous cell carcinoma cells is limited. In this study, we investigated apoptosis induced by EGCG and the underlying molecular mechanisms in human esophageal squamous cell carcinoma cells. The growth-inhibitory effects of EGCG on esophageal cancer cell (Eca109 and Ec9706) were detected by MTT. Using flow cytometry, we determined the cellular apoptosis, bcl-2, bax and caspase-3 protein expression in Eca109 and Ec9706 cells following treatment with EGCG for 24h. After treatment of Eca109/ABCG2 (an esophageal cancer multidrug resistance cell line) cells with adriamycin (ADM) combined with EGCG for 24h, the cellular apoptosis, mitochondrial membrane potential, ADM concentration in cells and ABCG2 protein expression were detected by flow cytometry. EGCG inhibited the growth of Eca109 and Ec9706 cells in a dose- and time- dependent manner. EGCG induced apoptosis, decreased the bcl-2 protein expression and increased the expression of bax and caspase-3 protein. The rate of apoptosis and ADM concentration in the Eca109/ABCG2 cells following treatment with ADM and EGCG were higher than that with ADM treatment alone, although the mitochondrial membrane potential was significantly lower (P<0.01). EGCG reduced the ABCG2 expression of Eca109/ABCG2 cells. Our data indicated that EGCG inhibited cell growth and induced esophageal cancer cell apoptosis. It reduced the bcl-2 protein expression and increased the bax and caspase-3 protein expression. EGCG reversed multi-drug resistance by reducing ABCG2 expression and increasing the anticancer drug concentration in cancer cells.

• Authors: Liang Liu, Yingchao Ju, Jing Wang, Rongmiao Zhou
• Journal: Pathology, research and practice (Pathol Res Pract) Vol. 213 Issue 10 Pg. 1242-1250 (Oct 2017) ISSN: 1618-0631 [Electronic] Germany
• PMID: 28964574 (Publication Type: Journal Article)
• Copyright: Copyright © 2017 Elsevier GmbH. All rights reserved.

Chemical References

• ABCG2 protein, human
• ATP Binding Cassette Transporter, Subfamily G, Member 2
• Antibiotics, Antineoplastic
• Antineoplastic Agents, Phytogenic
• Apoptosis Regulatory Proteins
• Neoplasm Proteins
• Doxorubicin
• Catechin
• epigallocatechin gallate

Topics

• ATP Binding Cassette Transporter, Subfamily G, Member 2 (antagonists & inhibitors, metabolism)
• Antibiotics, Antineoplastic (metabolism, pharmacology)
• Antineoplastic Agents, Phytogenic (pharmacology)
• Apoptosis (drug effects)
• Apoptosis Regulatory Proteins (metabolism)
• Carcinoma, Squamous Cell (drug therapy, metabolism, pathology)
• Catechin (analogs & derivatives, pharmacology)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Dose-Response Relationship, Drug
• Doxorubicin (metabolism, pharmacology)
• Drug Resistance, Multiple (drug effects)
• Drug Resistance, Neoplasm (drug effects)
• Esophageal Neoplasms (drug therapy, metabolism, pathology)
• Esophageal Squamous Cell Carcinoma
• Humans
• Membrane Potential, Mitochondrial (drug effects)
• Neoplasm Proteins (antagonists & inhibitors, metabolism)
• Signal Transduction (drug effects)
• Time Factors