Tibial
dyschondroplasia (TD) is one of the common skeletal abnormalities in fast-growing birds, and it is characterized by nonvascularized, unmineralized, and nonviable cartilage in the tibial growth plate that fails to form bone. The aim of this study was to check the in vitro effect of
apigenin and danshen on
heat-shock protein 90 (Hsp90) and
vascular endothelial growth factor (
VEGF) expressions in avian growth plate cells treated with sublethal concentration of
thiram. Initially, chondrocytes from chicken growth plates were isolated on culturx ed medium with and without various concentration of
thiram to determine the sublethal dose. Then, to check the effect of
apigenin and danshen, the chondrocytes were treated first with a sublethal (2.5 μM) concentration of
thiram and then with different doses (10, 20, 40, and 80 μM) of
apigenin and danshen. The
mRNA expression levels of Hsp90 and
VEGF genes were evaluated by quantitative reverse transcription polymerase chain reaction (RT-qPCR). The results showed that the expression levels of Hsp90 and
VEGF mRNA transcripts were increased significantly (P < 0.05) in
thiram-treated chondrocytes culture medium up to 1.5-fold, whereas
apigenin and danshen
therapy to chondrocytes in culture medium significantly (P < 0.05) reduced the Hsp90 and
VEGF expression levels. In conclusion, up-regulation of both (Hsp90 and
VEGF) genes and damage to chondrocytes in culture medium caused by
thiram can be restored by using
apigenin and danshen. Therefore,
apigenin and danshen
therapies are suggested and encouraged as a promising approach to control TD in broiler chickens.