Abstract | OBJECTIVE: To compare local and systemic profiles between different disease pathologies ( pneumonia and encephalitis) induced by influenza A virus (IAV). METHODS: An IAV pneumonia model was created by intranasal inoculation of C57BL/6 mice with influenza A/WSN/33 (H1N1) virus. Lung lavage and blood collection were performed on day 3 after IAV inoculation. Similarly, an IAV encephalitis mouse model was created by direct intracranial IAV inoculation. Cerebrospinal fluid (CSF) and blood collection were conducted according to the same schedule. Cytokine/ chemokine profiles were produced for each collected sample. Then the data were compared visually using radar charts. RESULTS: CONCLUSION: Investigating local samples such as BALF and CSF is important for evaluating local immune responses, providing insight into pathology at the primary loci of infection. Serum data alone might be insufficient to elucidate local immune responses and might not enable clinicians to devise the most appropriate treatment strategies.
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Authors | Yoshiharu Nagaoka, Nobuyuki Nosaka, Mutsuko Yamada, Masato Yashiro, Yosuke Washio, Kenji Baba, Tsuneo Morishima, Hirokazu Tsukahara |
Journal | Disease markers
(Dis Markers)
Vol. 2017
Pg. 2594231
( 2017)
ISSN: 1875-8630 [Electronic] United States |
PMID | 28912622
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Animals
- Bronchoalveolar Lavage Fluid
(immunology)
- Cytokines
(blood, cerebrospinal fluid)
- Encephalitis, Viral
(blood, cerebrospinal fluid, immunology)
- Influenza A Virus, H1N1 Subtype
(immunology)
- Mice
- Mice, Inbred C57BL
- Orthomyxoviridae Infections
(blood, cerebrospinal fluid, immunology)
- Pneumonia, Viral
(blood, cerebrospinal fluid, immunology)
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