Abstract |
We previously found that the novel histone deacetylase inhibitor (HDACI) butyroyloxymethyl diethylphosphate (AN-7) had greater selectivity against cutaneous T-cell lymphoma (CTCL) than SAHA. AN-7 synergizes with doxorubicin (Dox), an anthracycline antibiotic that induces DNA breaks. This study aimed to elucidate the mechanism underlying the effect of AN-7 on Dox-induced double-strand DNA breaks (DSBs) in CTCL, MyLa and Hut78 cell lines. The following markers/assays were employed: comet assay; western blot of γH2AX and p-KAP1; immunofluorescence of γH2AX nuclear foci; Western blot of repair protein; quantification of DSBs-repair through homologous recombination. DSB induction by Dox was evidenced by an increase in DSB markers, and DSBs-repair, by their subsequent decrease. The addition of AN-7 slightly increased Dox induction of DSBs in MyLa cells with no effect in Hut78 cells. AN-7 inhibited the repair of Dox-induced DSBs, with a more robust effect in Hut78. Treatment with AN-7 followed by Dox reduced the expression of DSB-repair proteins, with direct interference of AN-7 with the homologous recombination repair. AN-7 sensitizes CTCL cell lines to Dox, and when combined with Dox, sustains unrepaired DSBs by suppressing repair protein expression. Our data provide a mechanistic rationale for combining AN-7 with Dox or other DSB inducers as a therapeutic modality in CTCL.
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Authors | Lilach Moyal, Neta Goldfeiz, Batia Gorovitz, Ada Rephaeli, Efrat Tal, Nataly Tarasenko, Abraham Nudelman, Yael Ziv, Emmilia Hodak |
Journal | Investigational new drugs
(Invest New Drugs)
Vol. 36
Issue 1
Pg. 1-9
(02 2018)
ISSN: 1573-0646 [Electronic] United States |
PMID | 28884410
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibiotics, Antineoplastic
- Butyrates
- Histone Deacetylase Inhibitors
- Organophosphorus Compounds
- Prodrugs
- diethoxyphosphoryloxymethyl butanoate
- Doxorubicin
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Topics |
- Antibiotics, Antineoplastic
(pharmacology)
- Butyrates
(pharmacology)
- Cell Line, Tumor
- DNA Breaks, Double-Stranded
- DNA Repair
(drug effects)
- Doxorubicin
(pharmacology)
- Histone Deacetylase Inhibitors
(pharmacology)
- Humans
- Lymphoma, T-Cell, Cutaneous
(drug therapy)
- Organophosphorus Compounds
(pharmacology)
- Prodrugs
(pharmacology)
- Skin Neoplasms
(drug therapy)
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