Abstract |
Allergic conjunctivitis (AC), which is characterized by ocular itching, hyperemia, and edema, deteriorates quality of life. In this study, effects of anti-allergic drugs were evaluated by assessing eye-scratching behavior, the number of eosinophils in conjunctiva epithelial tissues, and concentrations of chemical mediators in the tears of the guinea pig model of ovalbumin (OA)-induced AC. METHODOLOGY: On day 0, 3-week-old guinea pigs were sensitized by OA subconjunctival injections. On days 15, 17, and 19, OA solution was administered. Anti-allergic eye drops were administered 5 and 15 min before the final OA challenge on day 19. Scratching behavior within 1 h after OA exposure was studied. Eosinophils in the conjunctiva were stained with Giemsa reagent. Histamine and substance P (SP) concentrations in tears were measured using ELISA. RESULTS: CONCLUSIONS: It is important to assess the anti-allergic AC drugs objectively because there are several of these drugs currently available. This model allows for an objective evaluation of anti-allergic drugs for AC.
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Authors | Yosuke Nakazawa, Mikako Oka, Makoto Takehana |
Journal | Open medicine (Warsaw, Poland)
(Open Med (Wars))
Vol. 12
Pg. 231-238
( 2017)
ISSN: 2391-5463 [Print] Poland |
PMID | 28848937
(Publication Type: Journal Article)
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