Dysregulation of
microRNA (
miRNA/miR) expression is causally associated with
cancer initiation and progression. However, the precise mechanisms by which dysregulated
miRNAs induce colorectal
tumorigenesis remain unknown. In the present study, downregulation of miR-34a was identified in
colorectal cancer cell lines and clinical specimens. Clinical studies revealed that miR-34a expression was negatively associated with distant
metastasis, and positively associated with differentiation and survival of human
colorectal cancer specimens. In vitro
miRNA functional assays demonstrated that miR-34a bound to the putative 3'-untranslated regions of Notch1 and Jagged1 in SW480 cells, and thereby attenuated the migration and invasion of the
colon cancer cells. It was additionally identified that miR-34a downregulated the expression of
vimentin and
fibronectin via Notch1 and Jagged1. Overall, these data indicate that miR-34a serves a key role in suppressing
colorectal cancer metastasis by targeting and regulating Notch signaling.