Hypoxia could enhance radioresistance in
prostate cancer cells through up-regulating HIF-1α, which could be inhibited by
statins in several
cancer cells. However, this effect of
statins in
prostate cancer remains unclear. In the present study, we aim to investigate the effect of
atorvastatin on HIF-1α expression and radiosensitivity in
prostate cancer cells. The
hypoxia-induced human
prostate cancer PC3 cells were generated by incubating with 5% O2 for 24 h. The cell viability and apoptosis were respectively analyzed by cell counting kit-8 (CCK-8) assay and flow cytometry. The HIF-1α
protein expression was assessed by Western blotting. HIF-1α expression in PC3 cells was significantly increased after incubating with 5% O2 for 24 h. The viability of
hypoxia-induced PC3 cells was inhibited by a higher dose of irradiation than control cells. The viability of
hypoxia-induced PC3 cells were inhibited by astorvastatin with a higher concentration than control cells. Astorvastatin reduced the HIF-1α
protein expression in
hypoxia-induced PC3 cells, and induced apoptosis of both control and
hypoxia-induced cells with and without irradiation.
Atorvastatin could enhance radiosensitivity in
hypoxia-induced
prostate cancer cells, which may be related with inhibition of HIF-1α
protein.