Bordetella pertussis causes
whooping cough, a severe and prolonged respiratory disease that results inhas high morbidity and mortality rates, particularly in developing countries. The number incidence of
whooping cough cases is increasing in many countries despite high
vaccine coverage. Causes for the re-emergence of the disease include the limited duration of protection conferred by the acellular
pertussis vaccines (aP)s and pathogenic adaptations that involve antigenic divergence from
vaccine strains. Therefore, current
vaccines therefore need to be improved. In the present study, we focused on five
autotransporters: namely SphB1, BatB, SphB2, Phg, and Vag8, which were previously found to be expressed by B. bronchiseptica during the course of
infection in rats and examined their protective efficiencies as
vaccine antigens. The passenger domains of these
proteins were produced in recombinant forms and used as
antigens. An intranasal murine challenge assay showed that immunization with a mixture of SphB1 and Vag8 (SV) significantly reduced bacterial load in the lower respiratory tract and a combination of aP and SV acts synergistically in effects of conferring protection against B.
pertussis infection, implying that these
antigens have potential as components to for improvinge th the currently available acellular
pertussis vaccine.