Abstract |
Free arachidonic acid (AA) is an important precursor of lipid mediators such as leukotrienes and prostaglandins that induces inflammation and is associated with atherosclerosis progression. Recent studies have shown that lysophosphatidylcholine acyltransferase-3 (LPCAT3) converts lysophosphatidylcholine (LPC) and free AA into phosphatidylcholine (PC)-containing AA (arachidonyl-PC) and thereby can regulate intracellular free-AA levels. However, the association between LPCAT3 and atherosclerosis remains to be established. In this study, we analyzed human and mouse atherosclerotic tissues to gain insight into the arachidonyl-PC metabolism involving LPCAT3 using imaging mass spectrometry. The data revealed a complementary distribution of arachidonyl-PC and LPC in human atherosclerotic tissues with arachidonyl-PC decreasing and LPC increasing as atherosclerosis progressed. Furthermore, we found a homologous distribution of LPCAT3 expression and arachidonyl-PC based on atherosclerotic progression. In contrast, in ApoE-deficient mice, atherosclerosis increased both arachidonyl-PC accumulation and LPCAT3 expression. Taken together, these findings suggest that the regulation of LPCAT3 expression might be associated with atherosclerotic progression in humans.
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Authors | Hiroki Tanaka, Nobuhiro Zaima, Takeshi Sasaki, Naoto Yamamoto, Kazunori Inuzuka, Tatsuro Yata, Takayuki Iwaki, Kazuo Umemura, Hideto Sano, Yuko Suzuki, Tetsumei Urano, Mitsutoshi Setou, Naoki Unno |
Journal | Journal of vascular research
(J Vasc Res)
Vol. 54
Issue 4
Pg. 200-208
( 2017)
ISSN: 1423-0135 [Electronic] Switzerland |
PMID | 28683445
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2017 S. Karger AG, Basel. |
Chemical References |
- Apolipoproteins E
- Lysophosphatidylcholines
- Phosphatidylcholines
- Arachidonic Acid
- 1-Acylglycerophosphocholine O-Acyltransferase
- LPCAT3 protein, human
- LPCAT3 protein, mouse
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Topics |
- 1-Acylglycerophosphocholine O-Acyltransferase
(metabolism)
- Aged
- Aged, 80 and over
- Animals
- Apolipoproteins E
(deficiency, genetics)
- Arachidonic Acid
(metabolism)
- Arteries
(enzymology, pathology)
- Atherosclerosis
(enzymology, genetics, pathology)
- Disease Models, Animal
- Disease Progression
- Female
- Humans
- Lysophosphatidylcholines
(metabolism)
- Male
- Mice, Inbred BALB C
- Mice, Knockout
- Middle Aged
- Muscle, Smooth, Vascular
(enzymology, pathology)
- Myocytes, Smooth Muscle
(enzymology, pathology)
- Phosphatidylcholines
(metabolism)
- Plaque, Atherosclerotic
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Up-Regulation
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