BCCIPβ modulates the ribosomal and extraribosomal function of S7 through a direct interaction.

Abstract

Extraribosomal functions of ribosomal proteins (RPs) have gained much attention for their implications in tumorigenesis and progression. However, the regulations for transition between the ribosomal and extraribosomal functions of RPs are rarely reported. Herein, we identified a ribosomal protein S7-interacting partner, BCCIPβ, which modulates the functional conversion of S7. Through the N-terminal acidic domain, BCCIPβ interacts with the central basic region in S7 and regulates the extraribosomal distribution of S7. BCCIPβ deficiency abrogates the ribosomal accumulation but enhances the ribosome-free location of S7. This translocation further impairs protein synthesis and triggers ribosomal stress. Consequently, BCCIPβ deficiency suppresses the ribosomal function and initiates the extraribosomal function of S7, resulting in restriction of cell proliferation. Moreover, clinically relevant S7 mutations were found to dampen the interaction with BCCIPβ and facilitate the functional transition of S7. In conclusion, BCCIPβ, as a S7 modulator, contributes to the regulation of ribosomal and extraribosomal functions of S7 and has implications in cell growth and tumor development.

• Authors: Qian Ba, Xiaoguang Li, Chao Huang, Junyang Li, Yijing Fu, Peizhan Chen, Juan Duan, Miao Hao, Yinghua Zhang, Jingquan Li, Chuanqi Sun, Hao Ying, Haiyun Song, Ruiwen Zhang, Zhiyuan Shen, Hui Wang
• Journal: Journal of molecular cell biology (J Mol Cell Biol) Vol. 9 Issue 3 Pg. 209-219 (06 01 2017) ISSN: 1759-4685 [Electronic] United States
• PMID: 28510697 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: © The Author (2017). Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS.

Chemical References

• BCCIP protein, human
• Calcium-Binding Proteins
• Cell Cycle Proteins
• Nuclear Proteins
• Ribosomal Proteins
• TP53 protein, human
• Tumor Suppressor Protein p53
• ribosomal protein S7

Topics

• Binding Sites
• Calcium-Binding Proteins (chemistry, genetics, metabolism)
• Cell Cycle Proteins (chemistry, genetics, metabolism)
• HEK293 Cells
• Humans
• Immunoprecipitation
• Mutation
• Neoplasms
• Nuclear Proteins (chemistry, genetics, metabolism)
• Protein Interaction Mapping
• Ribosomal Proteins (chemistry, genetics, metabolism)
• Ribosomes (metabolism)
• Signal Transduction
• Static Electricity
• Tumor Suppressor Protein p53 (metabolism)